LTbetaR:LIGHT[Biotinylated] Inhibitor Screening Assay Kit

The LTbetaR:LIGHT[Biotinylated] Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of LIGHT binding to LTbetaR . This kit comes in a convenient 96-well format, with biotin-labeled LIGHT, purified LTbetaR , streptavidin-labeled HRP, and assay buffer for 100 binding reactions. The key to this kit is the high sensitivity of detection of biotin-labeled LIGHT by streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, LTbetaR is coated on a 96-well plate. Next, biotinylated LIGHT is incubated with LTbetaR on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can be measured using a chemiluminescence reader.