Leucine Aminopeptidase (LAP) Activity Assay Kit

Detection principle LAP can catalyze the substrate L-leucine-4-nitroaniline to produce p-nitroaniline, which has the maximum absorption peak at the wavelength of 405 nm. The enzyme activity of LAP can be calculated by measuring the increasing OD value of the system. Performance characteristics Synonyms LAP Sample type Serum, plasma, animal tissue Sensitivity 5.2 U/L Detection range 5.2-201.8 U/L Detection method Colorimetric method Assay type Enzyme Activity Assay time 25 min Precision Average inter-assay CV: 6.4%Average intra-assay CV: 4.1% Other instruments required Micropipettor, Water bath, Centrifuge, Incubator, Vortex mixer Other reagents required Acetone Storage 2-8 °C Valid period 6 months Dilution of sample It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range ( 5.2-201.8 U/L). The recommended dilution factor for different samples is as follows (for reference only): Sample type Dilution factor Human serum 1 Dog serum 1 Rat serum 1 Cynomolgus monkey serum 1 10% Rat spleen tissue homogenate 1 10% Rat liver tissue homogenate 1 10% Rat kidney tissue homogenate 1 10% Rat lung tissue homogenate 1 Note: The diluent is reagent 1.