Lactulose Fluorometric Assay Kit

Detection principle: Lactulose (4-o-beta-galactopyranosyl-D-fructo-furanose), also known as galactoside fructose, 4-beta-D-galactosid-D-fructose and lactulose, contains one molecule of galactose and one molecule of fructose. Lactulose is a kind of synthetic disaccharide formed by base isomerization of lactose catalyzed by free amino group of casein during milk heat treatment. Lactulose can produce a specific product under the action of enzyme, which reacts with the chromogenic agent to produce a fluorescence product. The excitation wavelength is 530 nm, and the emission wavelength is 590 nm. Performance characteristics: Sample type: dairy product,feces Sensitivity: 4.78 µmol/L Detection range: 4.78-500 µmol/L Detection method: Fluorescence method Assay type: Quantitative Assay time: 150 min Precision: Average inter-assay CV: 7.4-9.7% Average intra-assay CV: 2.6-3.8% Other instruments required: Incubator(37?), Vortex mixer Storage: -20? Valid period: 12 months