L-Glutamine/Ammonia, UV method

Enzymatic method for the determination of L-glutamine and ammonia. Based on the spectrophotometric measurement of NADP+ formed through the combined action of glutaminase (GLT) and glutamate dehydrogenase (GlDH). This rapid and simple specific enzymatic method is used for the simultaneous determination of L-glutamine and ammonia (ammonium ions) in cell culture media and foodstuffs such as powdered dietary supplements, bakery products and fruit and vegetable products. L-Glutamine is an essential nutrient of cell culture media. However, the incorporation of this labile amino acid in growth media leads to its spontaneous break down to L-glutamate and free ammonium ions. The released ammonium ions are very toxic to cells. To overcome these issues, either pre-formulated growth media are used strictly within their recommended shelf-lives or L-glutamine is added just before use. In either case, monitoring of L-glutamine and ammonia is frequently performed both prior to, and during culturing. Related analytical kits are also available from Nzytech for ammonia (AK00091) and urea/ammonia (AK00101). Components: * Solution 1 (assay buffer 1) * Solution 2 (assay buffer 2) * Tablets 3 (cofactor NADPH) * Suspension 4 (enzyme GLT) * Suspension 5 (enzyme GlDH) * Solution 6 (Ammonia standard solution * 0.04 mg/mL) * Powder 7 (L-Glutamine control powder). Shipping Conditions: Room Temperature. Storage Conditions: 2 °C to 8 °C. Application: Fermentation, Food. Features: Detection limit: 0.07 mg/L ammonia, Detection limit: 0.54 mg/L L-glutamine, Range: 10-400 mg/L L-glutamine, Range: 10-70 mg/L ammonia. Detection Method: UV Method. Analyte: Ammonia, L-Glutamine