L-Arginine/Urea/Ammonia, UV method

Enzymatic method for the determination of L-arginine, urea and ammonia (Yeast Available Nitrogen -YAN). Based on the spectrophotometric measurement of NADPH consumed through the reactions, after addition of arginase (ARG), urease (URE) and glutamate dehydrogenase (GlDH). This rapid and simple stereo-specific enzymatic method is used for the simultaneous determination of L-arginine, urea and ammonia (yeast available nitrogen, YAN) in foodstuffs and in the wine industry (grape juice, fermenting must and fermenting wine). This kit is suitable for both manual and micro-volume formats. The determination of YAN (L-arginine, urea and ammonia) is an indicator of the amount of nitrogen available for yeast growth. YAN comprises three components: free ammonium ions, amino nitrogen from free amino acids and from the side chain of Larginine. As the concentrations of these compounds vary widely, each compound must be determined for the accurate calculation of YAN, which is possible by using this Nzytech's kit. In wine industry, the YAN can be managed by addition of nutrient supplements to grape juice prior to/during fermentation. Nzytech has also analytical kits for the determination of ammonia (AK000091) and both urea and ammonia (AK000101). Components: * Solution 1 (assay buffer) * Tablets 2 (cofactor NADPH) * Suspension 3 (enzyme GlDH) * Suspension 4. (enzume URE) * Suspension 5 (enzyme ARG) * Solution 6 (Ammonia standard solution- 0.04 mg/mL) * Powder 7 (L-Arginine standard). Shipping Conditions: Room Temperature. Storage Conditions: 2 °C to 8 °C. Application: Fermentation, Wine. Features: Detection limit: 0.07 mg/L ammonia, Detection limit: 0.13 mg/L urea, Detection limit: 0.37 mg/L L-arginine, Range: 10-70 mg/L ammonia, Range: 20-140 mg/L urea, Range: 50-400 mg/L L-arginine. Detection Method: UV Method. Analyte: Ammonia, L-Arginine, Urea