KAT8 (MOF) Chemiluminescence Assay Kit

The KAT8 (MOF) Chemiluminescence Assay Kit is an ELISA-based assay designed to measure the histone acetyl transferase activity of KAT8 (lysine acetyltransferase 8, also known as MOF) for screening and profiling applications. This kit comes in a convenient 96-well format with enough purified KAT8, acetyl donor, histone substrate, all the reagents necessary for assay detection, and blocking buffer for 96 enzyme reactions. Figure 1. KAT8 (MOF) Chemiluminescence Assay Kit schematic. KAT8 protein is added to a plate with a histone substrate in the presence of acetyl-CoA. After washing, an anti-acetyl-lysine antibody is added, followed by a secondary HRP-antibody. Lastly, HRP substrate is added and the chemiluminescence signal generated can be measured. The signal is proportional to the acetyltransferase activity of KAT8.Need us to run inhibitor screens or profile your compounds against KAT6B? Check out our Acethyltransferase Screening &Profiling Services.