KAT1 (HAT1) Chemiluminescent Assay Kit

The KAT1 (HAT1) Chemiluminescent Assay Kit is an ELISA-based assay designed to measure the histone acetyltransferase activity of HAT1 (histone acetyltransferase 1, also known as KAT1) for screening and profiling applications. This kit comes in a convenient 96-well format with enough purified KAT1, acetyl donor, pre-coated plate with histone substrate, all the reagents necessary for assay detection, and blocking buffer for 96 enzyme reactions. Figure 1. KAT1 (HAT1) Chemiluminescent Assay Kit schematic. KAT1 protein is added to a plate pre-coated with a histone substrate in the presence of acetyl-CoA. After washing, an anti-acetyl-lysine antibody is added, followed by a secondary HRP-antibody. Lastly, HRP substrate is added and the chemiluminescence signal generated can be measured. The signal is proportional to the acetyltransferase activity of KAT1. Need us to run inhibitor screens or profile your compounds against KAT1? Check out our Acethyltransferase Screening &Profiling Services.