IL-5: CD125 (IL-5RA) [Biotinylated] Inhibitor Screening Chemiluminescence Assay Kit

The IL-5: CD125 (IL-5RA) [Biotinylated] Inhibitor Screening Chemiluminescence Assay Kit is an ELISA-based assay designed to measure the binding between IL-5 (Interleukin-5) and CD125 (also known as IL-5RA) protein for screening and profiling applications. This kit comes with enough purified IL-5 (amino acids 20-134(end)) and biotinylated CD125 (amino acids 21-342), streptavidin-HRP, assay buffer, and detection reagent for 100 enzyme reactions. Figure 1. IL-5: CD125 (IL-5RA) [Biotinylated] Inhibitor Screening Assay Kit schematic. A 96-well plate is coated with IL-5 protein. After coating and blocking, biotinylated CD125 is added in an optimized assay buffer. Next, unbound biotinylated CD125 is washed away, and the plate is incubated with streptavidin-HRP. After a final wash, ELISA ECL substrate is added to produce chemiluminescence that can be measured using a chemiluminescence reader. The chemiluminescence signal is proportional to the efficacy of CD125 binding to IL-5. Need us to run inhibitor screens or profile your compounds against IL-5 binding to IL-5RA? Check out our Immunotherapy Screening Services.