IL-4RA: IL-4 [Biotinylated] Inhibitor Screening Chemiluminescence Assay Kit

The IL-4RA: IL-4 [Biotinylated] Inhibitor Screening Chemiluminescence Assay Kit is an ELISA-based assay designed to measure the binding between IL-4RA (interleukin 4 receptor alpha) and IL-4 protein for screening and profiling applications. This kit comes with enough purified recombinant IL-4RA (amino acids 26-232), biotinylated IL-4 (amino acids 25-153(end)), streptavidin-HRP, assay buffer, and detection reagent for 100 enzyme reactions. Figure 1. IL-4RA: IL-4 [Biotinylated] Inhibitor Screening Chemiluminescence Assay Kit schematic. A 96-well plate is coated with IL-4RA protein. After coating and blocking, biotinylated IL-4 is added in an optimized assay buffer. Next, unbound biotinylated IL-4 is washed away, and the plate is incubated with streptavidin-HRP. After a final wash, ELISA ECL substrate is added to produce chemiluminescence that can be measured using a chemiluminescence reader. The chemiluminescence signal is proportional to the efficacy of IL-4 binding to IL-4RA.Need us to run inhibitor screens or profile your compounds against IL-4RA binding to IL-4? Check out our Immunotherapy Screening Services.