IL-31: IL-31RA [Biotinylated] Inhibitor Screening Chemiluminescence Assay Kit

The IL-31: IL-31RA [Biotinylated] Inhibitor Screening Chemiluminescence Assay Kit is an ELISA-based assay designed to measure the binding between IL-31 and IL-31RA protein for screening and profiling applications. This kit comes with enough purified IL-31 (amino acids 24-164(end)) and biotinylated IL-31RA (amino acids 20-519), streptavidin-HRP, assay buffer, and detection reagent for 100 enzyme reactions. Figure 1. IL-31: IL-31RA [Biotinylated] Inhibitor Screening Chemiluminescence Assay Kit schematic. A 96-well plate is coated with IL-31 protein. After coating and blocking, biotinylated IL-31RA is added in an optimized assay buffer. Next, unbound biotinylated IL-31RA is washed away, and the plate is incubated with streptavidin-HRP. After a final wash, ELISA ECL substrate is added to produce chemiluminescence that can be measured using a chemiluminescence reader. The chemiluminescence signal is proportional to the efficacy of IL-31RA binding to IL-31. Need us to run inhibitor screens or profile your compounds that block the binding of IL31to IL31RA? Check out our Immunotherapy Biochemical Screening Services.