High-density Lipoprotein Cholesterol (HDL-C) Colorimetric Assay Kit (Double reagents)

Detection Principle: CM, VLDL and LDL coagulate in a polyanionic environment to form a polymer and are masked by the polymer. High-density lipoprotein (HDL) form soluble compounds under the action of a surfactant, so that HDL-C can directly react with enzyme reagents containing cholesterol esterase (CE) and cholesterol oxidase (CO) to produce hydrogen peroxide. Hydrogen peroxide is catalyzed by peroxidase (POD) in the presence of 4-aminoantipyrine (4-AA) and phenol (T-OOS) to form a red quinone compound,the shade is proportional to the amount of HDL-C. Synonyms HDL-C Sample Type Serum,Plasma Detection Method Colorimetric method Detection Instrument Microplate reader (530-570 nm, optimum wavelength: 546 nm) Research Area Metabolic Diseases, Glycolysis And Lipid Metabolism Other Reagents Required Normal saline (0.9% NaCl), PBS (0.01 mol/L, pH 7.4) Storage This product can be stored at 2-8°C for 12 months with shading light. Valid Period 12 months Sensitivity 0.06 mmol/L Detection Range 0.06-3.8 mmol/L Precision inter-assay CV: 0.05 , intra-assay CV: 0.03 Assay Time 30 min