Hexokinase(HK) Activity Assay Kit

Detection instrument: Microplate reader. Detection principle: HK converts glucose to glucose 6-phosphate, which is catalyzed by the enzyme. Meanwhile, NAD+ is reduced to NADH, which under the action of hydrogen transmitter, transfer electrons to WST-8 to produce the yellow product. The activity of HK can be calculated by measuring the change of absorbance value at 450 nm. Sample type: animal tissue and cell. Research area: Glycolysis & Carbohydrates. Sensitivity: 5.93 U/L. Detection range: 5.93-40.11 U/L. Recovery rate: 99. Inter CV: 9.1. Intra CV: 3.3