Helixyte Green(TM) Fluorimetric dsDNA Quantitation Kit *Optimized for Microplate Readers*

Ex: 501 nm. Em: 520 nm. Helixyte(TM) Green dsDNA Quantitation Assay Kit can be used for selectively detecting as little as 25 pg/ml of dsDNA in the presence of ssDNA, RNA, and free nucleotides. Helixyte(TM) Green exhibits large fluorescence enhancement upon binding to dsDNA. The assay is linear over three orders of magnitude and has little sequence dependence, allowing you to accurately measure DNA from many sources, including genomic DNA, viral DNA, miniprep DNA, or PCR amplification products. Helixyte(TM) Green dsDNA Quantitation Assay Kit is a few magnitudes more sensitive than UV absorbance readings. It is specific for dsDNA in the presence of equimolar amounts of RNA. The kit is robust with a mix and read format compatible with 96- and 384-well fluorescence-based microplate readers. It can also be used with a bench top fluorometer or a hand-held fluorescence meter (e.g., Qubit fluorometer).