Glycerol-3-phosphate (G3P) Fluorometric Assay Kit

Detection principle: Glycerol 3-phosphate (G3P), also known as 3-glycerophosphoic acid and glycerol alpha-phosphate, is mainly derived from glycolysis. G3P is an important intermediate in the glycerol-3-phosphate shuttle mechanism and studies have shown that G3P is involved in electron transport, and its metabolic level is related to lipid metabolism, insulin resistance and some cancers. The principle of this kit is that G3P is decomposed by enzymes, and its products generate fluorescent substances with probes under the action of other enzymes. The higher the fluorescence value, the higher the G3P content in the sample. Performance characteristics: Synonyms: G3P Sample type: tissue,cell Sensitivity: 0.19 µmol/L Detection range: 0.19-100 µmol/L Detection method: Fluorescence method Assay type: Quantitative Assay time: 30 min Precision: Average inter-assay CV: 4.6-10.0%Average intra-assay CV: 0.7-2.8% Other instruments required: Incubator(37?), 3 kD ultrafiltration tube Other reagents required: Normal saline (0.9% NaCl), Double distilled water Storage: -20? Valid period: 12 months