Enzolution(R) DHX9 ATPase TR-FRET Assay System

The Enzolution(R) DHX9 ATPase Assay System is intended for use with the Transcreener(R) ADP2 Assay Kits to measure the ATP-dependent RNA helicase activity of DHX9, a member of the a DExH/D helicase family. Though it is classified as an RNA helicase, DHX9 also has been reported to unwind DNA and DNA:RNA hybrids. ADP formation by the RNA helicase activity of DHX9 can be easily measured by the Transcreener ADP2 assay, a far-red, competitive fluorescence assay that enables single addition, mix-and-read detection in a continuous or endpoint format. The assay has been optimized and extensively validated for high throughput screening (HTS) and inhibitor dose response measurements using most multimode plate readers.