Enzolution(R) DDX5 ATPase TR-FRET Assay System

The Enzolution(R) DDX5 Assay System is intended for use with the Transcreener(R) ADP2 Assay Kits to measure the ATP-dependent RNA helicase activity of DDX5. DDX5, also known as p68, is a DEAD Box helicase protein characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), that catalyzes ATPdependent unwinding of RNA-RNA and RNA-DNA duplexes. ADP formation by the RNA helicase activity of DDX5 can be easily measured by the Transcreener ADP2 Assay, a far-red, competitive fluorescence assay that enables single addition, mix-and-read detection in a continuous or endpoint format. The assay has been optimized and extensively validated for high throughput screening (HTS) and inhibitor dose response measurements using most multimode plate readers. Protein Function: Involved in the alternative regulation of pre-mRNA splicing, its RNA helicase activity is necessary for increasing tau exon 10 inclusion and occurs in a RBM4-dependent manner. Binds to the tau pre-mRNA in the stem-loop region downstream of exon 10. The rate of ATP hydrolysis is highly stimulated by single-stranded RNA. Involved in transcriptional regulation, the function is independent of the RNA helicase activity. Transcriptional coactivator for androgen receptor AR but probably not ESR1. Synergizes with DDX17 and SRA1 RNA to activate MYOD1 transcriptional activity and involved in skeletal muscle differentiation. Transcriptional coactivator for p53/TP53 and involved in p53/TP53 transcriptional response to DNA damage and p53/TP53-dependent apoptosis. Transcriptional coactivator for RUNX2 and involved in regulation of osteoblast differentiation. Acts as a transcriptional repressor in a promoter-specific manner, the function probably involves association with histone deacetylases, such as HDAC1. As component of a large PER complex is involved in the inhibition of 3' transcriptional termination of circadian target genes such as PER1 and NR1D1 and the control of the circadian rhythms [The Uniprot Consortium]