DHX9 Helicase Assay Kit

The DHX9 Helicase Activity Assay Kit is a fluorogenic assay designed for screening and profiling DHX9 (DExH-Box Helicase 9) antagonists/inhibitors by monitoring their effect on DHX9-catalyzed RNA unwinding. DHX9 Helicase Activity Assay Kit comes in a convenient 96-well format, with contains enough purified recombinant DHX9 (amino acids 150-1150), ATP, DHX9 substrate, assay buffer and additives for 100 reactions. Figure 1: Illustration of the assay principle. The RNA probe is conjugated on one strand with the TAMRA (tetramethylrhodamine) fluorophore, and on the other strand with BHQ (Black Hole Quencher) which effectively quenches TAMRA fluorescence due to their proximity within the RNA double strand. DHX9 unwinding of the RNA probe separates the two strands, releasing TAMRA fluorescence. DHX9 activity, therefore, results in a proportional increase in fluorescence. Need us to run inhibitor screens or profile your compounds against DHX9 helicase activity? Check out our DNA Replication & Repair Screening Services. DHX9 (DExH-Box Helicase 9), also known as RNA Helicase A or Nuclear Helicase II (NDHII), is a member of the DEAH-containing family of RNA helicases. The encoded protein is an enzyme that catalyzes the ATP-dependent unwinding of double-stranded RNA and DNA-RNA complexes. This protein localizes mainly to the nucleus but can migrate to the cytoplasm, and functions as a transcriptional regulator. It is ubiquitously expressed and abundant. It interacts with many proteins, such as PRMT1 (protein arginine N-methyltransferase 1) and WRN (Werner Syndrome ATP-dependent Helicase. This protein may also be involved in the expression and nuclear export of retroviral RNAs, and is studied for its roles in cancer progression, antiviral immune response, and aging. DHX9 has become a relevant therapeutic target for diseases such as MM (multiple myeloma), showing promising results.