Delta 1 (Vdelta1) T Cell Expansion System

Delta 1 (Vdelta1) T Cell Expansion System is suitable for the ex vivo culture and expansion of human Vdelta1 (delta 1, D1) T cells. gammadelta T cells typically make up only 1-5% of total T cells in PBMCs (peripheral blood mononuclear cells) and therefore they need to be successfully activated and expanded to obtain an adequate number of cells for gammadelta T cell-based immunotherapy studies. This product contains media and reagents necessary to drive the robust activation and expansion of the Vdelta1 T cells subpopulation from PBMCs. Vdelta1 T Cell Expansion System is provided with enough reagents and materials for the ex vivo culture and expansion of human Vdelta1 T cells from a starting population of 3 x 107 PBMCs. It is possible to use this kit for multiple expansions from smaller PBMC amounts. Note: The use of this expansion system requires Anti-PE MicroBeads and MiniMACS(TM) Starting Kit (Miltenyi), that should be purchased separately (see information in Materials Required but not Supplied).