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| Item number | Size | Datasheet | Manual | SDS | Delivery time | Quantity | Price |
|---|---|---|---|---|---|---|---|
| BPS-83600 | 384 rxns | - | - |
3 - 15 business days* |
2,284.00€
|
If you have any questions, please use our Contact Form.
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
The DCAF16 Intrachain TR-FRET Assay Kit is a homogeneous, sensitive TR-FRET (Time-Resolved... more
Product information "DCAF16 Intrachain TR-FRET Assay Kit"
The DCAF16 Intrachain TR-FRET Assay Kit is a homogeneous, sensitive TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) assay kit designed to measure the auto-ubiquitination activity of E3 ligase complex DCAF16 (DDB1- and CUL4-associated factor 16). It utilizes a mix of donor and acceptor labeled ubiquitin to complete the TR-FRET pairing. This assay measures poly-ubiquitination since both the TR-FRET donor and acceptor are incorporated into poly-ubiquitin chains formed on DCAF16, making it suitable for real-time kinetics analyses of poly-ubiquitination. The kit contains enough purified DCAF16 complex, purified UBE1 and UbcH5b, detection reagents and assay buffer for 400 reactions. Figure 1: DCAF16 Intrachain TR-FRET Assay Kit schematic. The TR-FRET signal is proportional to DCAF16 auto-poly-ubiquitination activity. Need us to run inhibitor screens or profile your compounds against DCAF16? Check out our Ubiquitination Screening Services.
| Keywords: | DCAF1, Vpr-interacting protein, HIV-1 Vpr-binding protein, DDB1- and CUL4-associated factor 1, Serine/threonine-protein kinase VPRBP, DCAF, DCAF15, DDB1- and CUL4-associated factor 1, |
| Supplier: | BPS Bioscience |
| Supplier-Nr: | 83600 |
Properties
| Application: | Inhibitor screening, drug discovery, HTS, IC50 determination, real-time kinetics |
| Species reactivity: | human |
| Format: | Homog. |
Database Information
| KEGG ID : | K11789 | Matching products |
| UniProt ID : | Q9Y4B6 | Matching products |
| Gene ID : | GeneID 9730 | Matching products |
Handling & Safety
| Storage: | -80°C |
| Shipping: | -80°C (International: °C) |
Caution
Our products are for laboratory research use only: Not for administration to humans!
Our products are for laboratory research use only: Not for administration to humans!
Information about the product reference will follow.
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