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| Item number | Size | Datasheet | Manual | SDS | Delivery time | Quantity | Price |
|---|---|---|---|---|---|---|---|
| BPS-78892 | 384 rxns | - | - |
3 - 15 business days* |
2,099.00€
|
If you have any questions, please use our Contact Form.
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
The Dcaf11-driven p21 ubiquitination assay kit is a sensitive AlphaLisa(R) high-throughput... more
Product information "Dcaf11-Driven p21 Ubiquitination Assay Kit"
The Dcaf11-driven p21 ubiquitination assay kit is a sensitive AlphaLisa(R) high-throughput screening (HTS) assay kit, designed to measure Dcaf11 (DDB1- and Cul4-associated factor 11) E3 ligase activity in a homogeneous 384 reaction format. The assay kit comes with enough biotinylated ubiquitin, ATP, GST-tagged p21, assay buffer, detection buffer, purified UBE1 (E1), UbcH5b (E2), and Rbx1/Cul4B/Dcaf11/Ddb1 complex (E3) for 384 reactions. The assay detects mono-ubiquitination and poly-ubiquitination of p21. First, E1 and E2 enzymes are incubated with the E3 complex and with the target protein p21 (GST-tag) in the presence of biotin-conjugated ubiquitin and ATP. Ubiquitination of p21 occurs in a multistep ubiquitin transfer from E1 to E2 to E3, and E3-mediated conjugation of ubiquitin to p21. Next, glutathione-acceptor beads are added, followed by streptavidin-conjugated donor beads, and Alpha-counts are measured. The increase in Alpha-counts is proportional to the mono- or poly-ubiquitination of the GST-tagged p21.
| Keywords: | DDB1, XAP1, XPCe, DDBa, XAP-1, XPE-BF, UV-DDB 1, DDB p127 subunit, XPE-binding factor, HBV X-associated protein 1, DNA damage-binding protein 1, DNA damage-binding protein a, UV-damaged DNA-binding factor, UV-damaged DNA-binding protein 1, |
| Supplier: | BPS Bioscience |
| Supplier-Nr: | 78892 |
Properties
| Application: | Inhibitor screening, drug discovery, HTS, IC50 determinaion, Dcaf11-driven ubiquitination analysis |
| Species reactivity: | human |
Database Information
| KEGG ID : | K10610 | Matching products |
| UniProt ID : | Q16531 | Matching products |
| Gene ID | GeneID 1642 | Matching products |
Handling & Safety
| Storage: | -80°C |
| Shipping: | -80°C (International: °C) |
Caution
Our products are for laboratory research use only: Not for administration to humans!
Our products are for laboratory research use only: Not for administration to humans!
Information about the product reference will follow.
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