DCAF1 Intrachain TR-FRET Assay Kit

DCAF1 Intrachain TR-FRET Assay Kit
Item number Size Datasheet Manual SDS Delivery time Quantity Price
BPS-82517 384 rxns - -

3 - 15 business days*

2,341.00€
 
The DCAF1 Intrachain TR-FRET Assay Kit is a homogeneous, sensitive TR-FRET (Time-Resolved... more
Product information "DCAF1 Intrachain TR-FRET Assay Kit"
The DCAF1 Intrachain TR-FRET Assay Kit is a homogeneous, sensitive TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) assay kit designed to measure DCAF1 (DDB1 and CUL4-associated Factor 1) complex auto-ubiquitination activity. It utilizes a mix of donor and acceptor labeled ubiquitin to complete the TR-FRET pairing. This assay measures poly-ubiquitination since both the TR-FRET donor and acceptor are incorporated into poly-ubiquitin chains formed on DCAF1. The kit contains enough purified DCAF1/Rbx1 (M5I)/DDB1/CUL4A Complex, purified UBE1 and UbcH5b, detection reagents and assay buffer for 400 reactions.
Keywords: VprBP, KIAA0800, EC=2.7.11.1, Vpr-interacting protein, HIV-1 Vpr-binding protein, DDB1- and CUL4-associated factor 1, Serine/threonine-protein kinase VPRBP,
Supplier: BPS Bioscience
Supplier-Nr: 82517

Properties

Application: DCAF1 E3 ligase inhibitor screening, drug discovery, HTS, IC50 determination, real-time kinetics
Species reactivity: human
Format: Homog.

Handling & Safety

Storage: -80°C
Shipping: -80°C (International: °C)
Caution
Our products are for laboratory research use only: Not for administration to humans!
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