Cell Navigator(TM) Flow Cytometric Exosome Staining Kit

Correction Factor (280 nm) 0.35. Extinction coefficient (cm^-1 M^-1): 73000. Excitation (nm): 491. Emission (nm): 516. Quantum yield 0.92. Exosomes, nanoscale (30-150 nm) membrane-bound vesicles secreted by cells, are central to intercellular communication. They transfer proteins, lipids, and nucleic acids, including RNA species, between cells, playing a pivotal role in physiological and pathological processes such as immune modulation, disease progression, and genetic information exchange. This makes exosomes key targets in therapeutic and diagnostic research. However, challenges such as dye aggregation and the absence of bright, specific probes have hindered the reliable detection and analysis of exosomes. The Cell Navigator(TM) Flow Cytometric Exosome Staining Kit addresses these challenges by enabling the detection of CD9-positive exosomes in purified or bead-bound samples. It utilizes a fluorescent dye-conjugated CD9 marker for targeted visualization, providing a straightforward staining protocol for rapid and accurate exosome detection in diverse sample matrices, including cell culture supernatants, biological fluids, and isolated exosome preparations. To ensure effective detection and analysis, it is essential to enrich the cell culture media for exosomes before staining. This can be achieved through efficient methods such as the ReadiPrep(TM) Exosome Isolation Kit (Cat No. ABD-60204) or traditional ultracentrifugation. Platform: Flow cytometer, Excitation 488 nm, Laser Emission 530/30 nm. Filter Instrument specification(s): FITC Channel.