Cell Meter(TM) Fluorimetric Intracellular Nitric oxide (NO) Activity Assay Kit *Red Fluorescence Opt

Ex: 630 nm. Em: 660 nm. Nitric oxide (NO) is an important biological regulator involved in numbers of physiological and pathological processes. Altered NO production is implicated in various immunological, cardiovascular, neurodegenerative and inflammatory diseases. As a free radical, NO is rapidly oxidized and there is relatively low concentrations of NO existing in vivo. It has been challenging to detect and understand the role of NO in biological systems. Cell Meter(TM) Fluorimetric Intracellular Nitric Oxide Assay Kit provides a sensitive tool to monitor intracellular NO level in live cells. Nitrixyte(TM) probes are developed and used in our kit as an excellent replacement for DAF-2 for the detection and imaging of free NO in cells. Compared to the commonly used DAF-2 probe, Nitrixyte(TM) probes have better photostability and enhanced cell permeability. This particular kit uses Nitrixyte(TM) Red that can react with NO to generate a bright red fluorescent product that has spectral properties similar to Texas Red(R). Nitrixyte(TM) Red can be readily loaded into live cells, and its fluorescence signal can be conveniently monitored using the filter set of Red. This kit is optimized for flow cytometry applications.