Cell Meter(TM) 2-NBDG Glucose Uptake Assay Kit

Ex: 485 nm. Em: 540 nm. Soluble in: DMSO. Glucose metabolism, a process which converts glucose into energy, is a primary source of energy supply in most organisms. 2-NBDG [2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose], a fluorescently tagged glucose tracer, has been proven to effectively monitor glucose transportation in cells, as 2-NBDG transports into cells by the same glucose transporters (GLUTs) as glucose. Once 2-NBDG is uptaken in cells, it undergoes phosphorylation at C-6 position to give 2-NBDG-6-phosphate, which is well retained within the cells. Compared to other glucose tracers, such as 2-DG or FDG, 2-NBDG allows in situ measurements of 2-NBDG with high temporal and spatial resolution at single cell level. AAT Bioquest's Cell MeterT 2-NBDG Glucose Uptake Assay Kit provides a sensitive and non-radioactive assay for measuring glucose uptake in cultured cells. In this kit, Assay Buffer I is used to enhance the uptake and retention of 2-NBDG in cells, while Assay Buffer II can improve the signal-to-background ratio of 2-NBDG in the cells. The fluorescence signal can be monitored by fluorescence microscope or flow cytometer with a FITC filter set. Cell MeterT 2-NBDG Glucose Uptake Assay Kit is the most robust tool for monitoring glucose transporters.