CD200R1:CD200 [Biotinylated] Inhibitor Screening Chemiluminescence Assay Kit

The CD200R1:CD200 [Biotinylated] Inhibitor Screening Chemiluminescence Assay Kit is an ELISA designed to assess the efficacy of inhibitors of the binding between CD200R1 (CD200 receptor 1) and CD200 (cluster of differentiation 200) protein for screening and profiling applications. This kit comes with enough purified CD200R1 (amino acids 29-265) and biotinylated CD200 (amino acids 31-232), streptavidin-HRP, assay buffer, and detection reagent for 100 enzyme reactions. A 96-well plate is coated with CD200R1 protein. After blocking, biotinylated CD200 is added in an optimized assay buffer. Next, unbound biotinylated CD200 is washed away, and the plate is incubated with streptavidin-HRP. After a final wash, ELISA ECL substrate is added to produce chemiluminescence that can be measured using a chemiluminescence reader. The chemiluminescence signal is proportional to CD200 binding to CD200R1. Need us to run inhibitor screens or profile your compounds against IL-17RA:IL-17A[Biotin]? Check out our Immunotherapy Biochemical Screening Services.