CD200:CD200R1 [Biotinylated] Inhibitor Screening Chemiluminescence Assay Kit

The CD200:CD200R1 [Biotinylated] Inhibitor Screening Chemiluminescence Assay Kit is designed for screening and profiling molecules that block the binding between CD200 (cluster of differentiation 200) and CD200R1 (CD200 receptor 1). This kit comes in a convenient 96-well format, with enough recombinant human biotin-labeled CD200R1 (amino acids 29-265), human CD200 (amino acids 31-232), streptavidin-HRP, and assay buffer for 100 binding reactions. CD200 is coated onto a 96-well plate. After blocking, the plate is pre-incubated with an inhibitor or neutralizing antibody. After incubation with Biotin-CD200R1, the plate is washed and Streptavidin-HRP is added. After the  ELISA ECL substrate is added  the resulting signal can be measured using a chemiluminescence microplate reader. The chemiluminescence signal is proportional to the binding of CD200 to CD200R1.