Ca2+-ATPase Activity Assay Kit

Detection instrument: Microplate reader. Detection principle: ATPase can decompose ATP to produce inorganic phosphorus. The activity of ATPase can be expressed by measuring the production amount of inorganic phosphorus in unit time. In the control system, Ca2+ -ATPase activity was inhibited, while in the sample system, Ca2+ -ATPase activity was not inhibited. The difference of inorganic phosphorus content between the sample and the control was the inorganic phosphorus produced by Ca2+ -ATPase during the incubation time. The activity of Ca2+ -ATPase was determined by inorganic phosphorus production. Sample type: animal tissue. Research area: Enzymes,Oxidative stress. Assay time: 90 min. Sensitivity: 1.18 U/kg wet weight. Detection range: 1.18-286.43 U/kg wet weight. Recovery rate: 107. Inter CV: 8. Intra CV: 6