c-Myc: WDR5 [Biotinylated] Binding Chemiluminescent Assay Kit

The c-Myc: WDR5 [Biotinylated] Binding Chemiluminescent Assay Kit is an ELISA designed to measure the binding between c-Myc and WDR5 (WD40 repeat domain 5) protein for screening and profiling applications. This kit comes with enough purified c-Myc (amino acids 2-454(end)) and biotinylated WDR5 (amino acids 2-334(end)), MLL1 (amino acids 3745-end), streptavidin-HRP, assay buffer, and detection reagent for 100 enzyme reactions. Figure 1. c-Myc: WDR5 [Biotinylated] Binding Chemiluminescent Assay Kit schematic. A 96-well plate is coated with c-Myc protein. After coating and blocking, MLL1 and biotinylated WDR5 are added in an optimized assay buffer. Next, unbound biotinylated WDR5-MLL complexes are washed away, and the plate is incubated with streptavidin-HRP. After a final wash, ELISA ECL substrate is added to produce chemiluminescence that can be measured using a chemiluminescence reader. The chemiluminescence signal is proportional to the efficacy of WDR5 binding to c-Myc. Interested in having us perform the screening and profiling of inhibitors or blocking antibodies? Visit our Methyltransferase Screening& Profiling Services