Biuret Protein Colorimetric Assay Kit

Detection principle Any compound that contains two -CONH2 in the molecule can react with alkaline copper solution to form a purple complex, which is known as the biuret reaction. Many peptide bonds (-CONH-) in protein molecules can perform this reaction, and the color degree of all kinds of proteins are essentially the same. Performance characteristics Synonyms TP Sample type Serum,plasma,tissue Sensitivity 0.58 g/L Detection range 0.58-100 g/L Detection method Colorimetric method Assay type Quantitative Assay time 20 min Precision Average inter-assay CV: 6.5%Average intra-assay CV: 4% Other instruments required Micropipettor, Incubator, Vortex mixer, Centrifuge Other reagents required Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4) Storage Reagent 3: -20 °C, others: 2-8 °C Valid period 6 months Dilution of sample It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.58-100 g/L). The recommended dilution factor for different samples is as follows (for reference only): Sample type Dilution factor Human serum 1 Human plasma 1 Rat serum 2-4 Mouse plasma 1 Rabbit serum 1 Chicken plasma 1 Horse serum 1-3 Porcine serum 1-3 Dog serum 2-4 10% Rat spleen tissue homogenate 1 10% Mouse liver tissue homogenate 1 10% Mouse kidney tissue homogenate 1 Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).