Amplite(TM) Rapid Colorimetric Protein Aldehyde Content Quantitation Kit

Site-selective modification of proteins is of major interest in chemical biology. Most of the traditional methods used for protein modifications are based on reactions of amine or thiol groups within a protein. However, many methods have been developed to incorporate aldehyde functional groups into proteins. Rapid and accurate measurement of aldehyde content is an important tool for biological and chemical research. Amplite(TM) Rapid Colorimetric Protein Aldehyde Content Quantitation Kit provides an accurate method to quantify aldehyde groups using our proprietary AldeView(TM) 488 sensor, which has the maximum absorbance at ~495nm. AldeView(TM) 488 reacts with the aldehyde-modified protein samples. The resulted product is run through a single spin column to remove the excess AldeView(TM) 488 sensor. The absorbance spectra of purified product are measured, and the aldehyde to protein ratio can be determined via the absorbance ratio of 495 nm/280 nm. Amplite(TM) Rapid Colorimetric Protein Aldehyde Content Quantitation Kit can be performed in a traditional cuvette, NanoDrop(TM) Spectrophotometer or a convenient 96-well absorbance plate reader with a UV-transparent plate.