Alkaline Phosphatase (ALP) Activity Assay Kit (PNPP method)

Detection principle Alkaline phosphatase (ALP) is a group of cytomembrane-related enzymes with hydrolysis and transfer activity, acting on a variety of phosphate substrates. ALP is a homologous dimerase and each catalytic site contains three metal ions. There are four isozymes in humans: tissue nonspecific ALP, intestinal ALP, placental ALP and genital cell ALP. Performance characteristics Synonyms: ALP Sample type: Serum, plasma, animal tissue, cells Sensitivity: 0.27 U/L Detection range: 0.27-50.8 U/L Detection method: Colorimetric method Assay type: Enzyme Activity Assay time: 30 min Precision: Average inter-assay CV: 4.6%Average intra-assay CV: 1.2% Other instruments required: Micropipettor, Water bath, Incubator Other reagents required: Normal saline (0.9% NaCl) Storage: -20? Valid period: 6 months Dilution of sample It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.27-50.8 U/L). The recommended dilution factor for different samples is as follows (for reference only): Sample type Dilution factor Human serum 10-20 Mouse serum 20-30 Rat serum 10-20 Mouse plasma 10-20 HepG2 supernatant 8-12 10% Rat kidney tissue homogenate 400-1200 10% Mouse liver tissue homogenate 10-15 10% Rat lung tissue homogenate 50-100 10% Mouse brain tissue homogenate 30-50 Human urine 1 Note: The diluent is reagent 1.