Aldehyde Dehydrogenase (ALDH) Fluorometric Activity Assay Kit

Detection instrument: Fluorescence microplate reader. Detection principle: The Detection principle: of this kit is that the substrate under the action of aldehyde dehydrogenase transforms NAD+ into NADH, NADH reacts with the fluorescent probe to form fluorescent substance under the action of enzymes. The activity of ALDH can be calculated by measuring the fluorescence intensity at the excitation wavelength of 535 nm and the emission wavelength of 587 nm. Sample type: serum (plasma), animal tissue, cell. Research area: Liver biomarkers. Assay time: 20 min. Sensitivity: 0.06 µmol/L. Detection range: 0.06-3 µmol/L. Recovery rate: 100. Inter CV: 8.4. Intra CV: 4