Alanine Aminotransferase (ALT/GPT) Activity Fluorometric Assay Kit

Detection principle ALT catalyze the amino conversion reaction between alanine and alpha-ketoglutaric acid to produce pyruvic acid and glutamic acid. Under the action of pyruvate oxidase, pyruvic acid generates H2O2, which reacts with the non-fluorescent substance to form fluorescent substance under the action of peroxidase. The activity of ALT can be calculated by measuring the increase of fluorescence value at the excitation wavelength of 535 nm and the emission wavelength of 590 nm. Performance characteristics Synonyms ALT Sample type Serum, plasma, animal tissue Sensitivity 0.01 U/L Detection range 0.01-0.83 U/L Detection method Fluorescence method Assay type Enzyme Activity Assay time 75 min Precision Average inter-assay CV: 9.800000000000001%Average intra-assay CV: 2.3% Other instruments required Pipettor, Vortex mixer, Centrifuge Storage -20 °C Valid period 6 months Dilution of sample It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.01-0.83 U/L). The recommended dilution factor for different samples is as follows (for reference only): Sample type Dilution factor Human serum 10-15 Dog serum 5-10 Rat serum 10-15 10% Mouse heart tissue homogenate 100-120 10% Rat spleen tissue homogenate 10-15 10% Rat liver tissue homogenate 300-500 10% Rat kidney tissue homogenate 100-120 10% Rat lung tissue homogenate 100-120 Note: The diluent is reagent 1.