Adenosine Deaminase (ADA) Activity Assay Kit

Detection principle Adenosine deaminase (ADA) can hydrolyzed the substrate adenosine to form hypoxanthine riboside, which is hydrolyzed by purine riboside phosphatase to produce hypoxanthine and phosphate ribose. Under the action of xanthine oxidase, hypoxanthine produces hydrogen peroxide, which produces red substance under the action of peroxidase, 4-aminotepyrine and color source. The red substance has the maximum absorption peak at 550 nm and the changes of absorbance is proportional to the activity of ADA. Performance characteristics Synonyms ADA Sample type serum, plasma, animal tissue Sensitivity 0.03 U/L Detection range 0.03-99 U/L Detection method Colorimetric method Assay type Enzyme Activity Assay time 40 min Precision Average inter-assay CV: 6%Average intra-assay CV: 3% Other instruments required Micropipette, Centrifuge, Water bath/Incubator Other reagents required Normal saline (0.9% NaCl) Storage 2-8 °C Valid period 6 months Dilution of sample It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.03-99 U/L). The recommended dilution factor for different samples is as follows (for reference only): Sample type Dilution factor 10% Mouse liver tissue homogenate 1 10% Rat kidney tissue homogenate 1 10% Rat heart tissue homogenate 2-3 10% Rat spleen tissue homogenate 2-3 Mouse serum 1 Human serum 1 Note: The diluent is normal saline (0.9% NaCl).