ADAR1:RNA TR-FRET Assay Kit

The ADAR1:RNA TR-FRET Assay Kit is designed to measure the binding of ADAR1 (adenosine deaminase, RNA-specific 1) to RNA for screening and profiling applications using TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer). It utilizes Terbium-labeled donor and dye-labeled acceptor to complete the TR-FRET pairing. The ADAR1:RNA TR-FRET Assay Kit comes in a convenient 384-well format, with enough purified ADAR1, Tb-Labeled Donor and Dye-Labeled Acceptor, RNase inhibitor, ADAR1 substrate and assay buffer for 384 reactions. The assay also includes a Competitor RNA as control. Figure 1: ADAR1 mechanism of action on RNA molecules. Figure 2: Assay principle. The Terbium-labeled anti-FLAG antibody donor binds to FLAG-tagged ADAR1, while the dye-labeled streptavidin acceptor binds to the biotinylated RNA substrate. When ADAR1 forms a complex with its RNA substrate TR-FRET occurs and can be measured using a TR-FRET fluorescence plate reader. TR-FRET signal is proportional to RNA binding.