Biochemical Assays

A biochemical assay is an analytical method for quantifying the activity of a biological molecule or a cellular process. The assays provide valuable insights into metabolic reactions and the mechanisms of action of biomolecules such as enzymes and proteins. In addition, biochemical assays can be used to identify and characterize protein-protein, protein-RNA, and protein-DNA interactions. They are used in basic as well as in applied research and represent a helpful tool for characterizing drugs in the pharmaceutical industry.

A biochemical assay is an analytical method for quantifying the activity of a biological molecule or a cellular process. The assays provide valuable insights into metabolic reactions and the... read more »
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Biochemical Assays

A biochemical assay is an analytical method for quantifying the activity of a biological molecule or a cellular process. The assays provide valuable insights into metabolic reactions and the mechanisms of action of biomolecules such as enzymes and proteins. In addition, biochemical assays can be used to identify and characterize protein-protein, protein-RNA, and protein-DNA interactions. They are used in basic as well as in applied research and represent a helpful tool for characterizing drugs in the pharmaceutical industry.

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Cholesterol Fluorometric Assay Kit
Cholesterol Fluorometric Assay Kit

Item number: Cay10007640-96

Cholesterol, particularly in the form of low density lipoproteins (LDL), is well understood to be associated with increased risk of coronary heart disease. The measurement of cholesterol is one of the most common tests performed in the clinical laboratory setting. However, simple and easy assays for cholesterol in...
Application: Cholesterol quantification
From 458.00€ *
Review
Glycerol Colorimetric Assay Kit
Glycerol Colorimetric Assay Kit

Item number: Cay10010755-96

Glycerol is the backbone of triglycerides and an important intermediate in energy metabolism being involved in both oxidative and synthetic processes. The measurement of circulating levels of glycerol and free fatty acids are considered to reflect lipolysis and are therefore useful parameters to evaluate in various...
Application: Glycerol detection
483.00€ *
Review
COX Fluorescent Inhibitor Screening Assay Kit
COX Fluorescent Inhibitor Screening Assay Kit

Item number: Cay700100-192

Cyclooxygenase (COX, also called Prostaglandin H Synthase or PGHS) is a bifunctional enzyme exhibiting both COX and peroxidase activities. The COX component converts arachidonic acid to a hydroperoxy endoperoxide (PGG2), and the peroxidase component reduces the endoperoxide to the corresponding alcohol (PGH2), the...
Keywords: Cyclooxygenase Fluorescent Inhibitor Screening Assay Kit
Application: COX inhibitor screening
1,062.00€ *
Review
NF-kappaB (p65) Transcription Factor Assay Kit
NF-kappaB (p65) Transcription Factor Assay Kit

Item number: Cay10007889-96

Cayman's NF-kappaB (p65) Transcription Factor Assay is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity in nuclear extracts and whole cell lysates. A 96-well enzyme-linked immunosorbent assay (ELISA) replaces the cumbersome radioactive electrophoretic mobility...
Keywords: RELA, NFKB3, Transcription factor p65, Nuclear factor NF-kappa-B p65 subunit, Nuclear factor of kappa light polypeptide...
Application: NF-kappaB binding detection
Species reactivity: human, mouse, rat
834.00€ *
Review
Amplite(TM) Fluorimetric Lysyl Oxidase Assay Kit *Red Fluorescence*
Amplite(TM) Fluorimetric Lysyl Oxidase Assay Kit *Red...

Item number: ABD-15255

This kit offers a sensitive fluorescent assay for lysyl oxidase activity that utilizes 1,5-diaminopentane as substrate, and released hydrogen peroxide is detected using our Amplite HRP substrate in HRP-coupled reactions.
Keywords: EC=1.4.3.13
Application: Lysyl oxidase activity assay
434.00€ *
Review
COX-2 (human) Inhibitor Screening Assay Kit
COX-2 (human) Inhibitor Screening Assay Kit

Item number: Cay701080-96

The COX-2 (human) Inhibitor Screening Assay directly measures PGF2alpha by stannous chloride (SnCl2) reduction of COX-derived PGH2 produced in the COX reaction. The prostanoid product is quantified via ELISA using an antiserum specific to PGF2alpha. This assay includes recombinant human COX-2 and is an excellent...
Keywords: Cyclooxygenase 2
Application: Inhibitor screening
762.00€ *
Review
Amplite(TM) Fluorimetric Proteasome 20S Activity Assay Kit *Green Fluorescence*
Amplite(TM) Fluorimetric Proteasome 20S Activity Assay...

Item number: ABD-13456

Our Amplite(TM) Fluorometric 20S Proteasome Assay Kit is a homogeneous fluorescent assay that measures the chymotrypsin-like protease activity associated with the proteasome complex either in cultured cells or cell lysates. This kit uses LLVY-R110 as a fluorogenic indicator for proteasome activity. Cleavage of...
Keywords: LLVY-R110, chymotrypsin-like protease activity
Application: Proteasome 20S activity assay, HTS
434.00€ *
Review
L-Lactate Assay Kit
L-Lactate Assay Kit

Item number: Cay700510-96

Cayman's L-Lactate Assay provides both a fluorescence and an absorbance-based method for detecting L-lactate in biological samples such as serum, plasma, blood, urine, and saliva. It can also be utilized to determine intracellular and extracellular lactate concentrations in cell culture samples. In the assay, LDH...
Application: Fluorescence-based L-lactate detection
651.00€ *
Review
Amplite(TM) Fluorimetric Glutathione GSH/GSSG Ratio Assay Kit *Green Fluorescence*
Amplite(TM) Fluorimetric Glutathione GSH/GSSG Ratio Assay...

Item number: ABD-10056

When cells are exposed to increased levels of oxidative stress, GSSG will accumulate and the ratio of GSH to GSSG will decrease. The glutathione redutase recycles GSSG to GSH with simultaneous oxidation of b-nicotinamide adenine dinuclecotide phosphate. The monitoring of GSH/GSSG ratio and the quantification of GSSG...
Application: GSH quantification, Redox status analyses
434.00€ *
Review
S-Nitrosylated Protein Detection Kit (Biotin Switch)
S-Nitrosylated Protein Detection Kit (Biotin Switch)

Item number: Cay10006518-1

Nitric oxide (NO) is produced by three distinct isoforms of nitric oxide synthase and functions as a key signaling molecule in physiology and pathophysiology. NO transduces its effects by reacting either directly with heme and non-heme centers of proteins or indirectly via further oxidation to various reactive...
Keywords: S-NO
Application: S-nitrosylated protein detection, WB
590.00€ *
Review
NEW
Amylin 3 Receptor (human) Reporter Assay Kit
Amylin 3 Receptor (human) Reporter Assay Kit

Item number: Cay502902-1

Cayman's reverse transfection reporter assays have overcome many of the disadvantages of other transfection approaches. In this method, a proprietary transfection complex containing DNA and an optimized mixture of lipids and proteins has been evenly applied and processed on the culture surface of multi-well plates....
Keywords: AMY3 Receptor, AMY3R, CTR/RAMP3
Application: Chemiluminescent cell-based SEAP reporter assay
711.00€ *
Review
NEW
Calcitonin Receptor (human) Reporter Assay Kit
Calcitonin Receptor (human) Reporter Assay Kit

Item number: Cay502903-1

Cayman's reverse transfection reporter assays have overcome many of the disadvantages of other transfection approaches. In this method, a proprietary transfection complex containing DNA and an optimized mixture of lipids and proteins has been evenly applied and processed on the culture surface of multi-well plates....
Keywords: CTR
Application: Chemiluminescent cell-based SEAP reporter assay
711.00€ *
Review
3 from 240 pages