Biochemical Assays

A biochemical assay is an analytical method for quantifying the activity of a biological molecule or a cellular process. The assays provide valuable insights into metabolic reactions and the mechanisms of action of biomolecules such as enzymes and proteins. In addition, biochemical assays can be used to identify and characterize protein-protein, protein-RNA, and protein-DNA interactions. They are used in basic as well as in applied research and represent a helpful tool for characterizing drugs in the pharmaceutical industry.

A biochemical assay is an analytical method for quantifying the activity of a biological molecule or a cellular process. The assays provide valuable insights into metabolic reactions and the... read more »
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Biochemical Assays

A biochemical assay is an analytical method for quantifying the activity of a biological molecule or a cellular process. The assays provide valuable insights into metabolic reactions and the mechanisms of action of biomolecules such as enzymes and proteins. In addition, biochemical assays can be used to identify and characterize protein-protein, protein-RNA, and protein-DNA interactions. They are used in basic as well as in applied research and represent a helpful tool for characterizing drugs in the pharmaceutical industry.

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Caspase 9 Activity Assay Kit (Colorimetric Method)
Caspase 9 Activity Assay Kit (Colorimetric Method)

Item number: E-CK-A389.100

Caspase 9, also known as ICE-LAP6 or Mch6, is a relatively upstream caspase in the process of apoptosis signal transduction. After mitochondria release cytochrome c, caspase 9 can form complex with cytochrome c and Apaf1 and can be activated. Activated caspase 9 can activate caspase 3, the most critical enzyme of...
Keywords: ICE-LAP6, Mch6, CASP9 activity, photometric assay
Application: Spectrophotometer, enzyme assay
Species reactivity: broad
From 69.00€ *
Review
Pyruvate Decarboxylase (PDC) Activity Assay Kit
Pyruvate Decarboxylase (PDC) Activity Assay Kit

Item number: E-BC-K500-M.48

Pyruvate decarboxylase (PDC) mainly exists in yeast and is one of the key enzymes in ethanol fermentation. PDC catalyzes pyruvate decarboxylate to acetaldehyde, which reacts under the action of ethanol dehydrogenase (ADH), and catalyzes NADH to convert into NAD+. NADH has a characteristic absorption peak at 340 nm....
From 284.00€ *
Review
Glutamic Acid Colorimetric Assay Kit
Glutamic Acid Colorimetric Assay Kit

Item number: E-BC-K118-S.100

Detection principle Glutamic acid can react with NAD+ under the catalysis of glutamate dehydrogenase to produce alpha-ketoglutaric acid, NADH and NH4+. NADH has the maximum absorption at 340 nm. And glutamic acid content can be calculated by measuring the change of NADH. Performance characteristics Sample type...
Application: Spectrophotometer
From 203.00€ *
Review
BCL-2 TR-FRET Assay Kit
BCL-2 TR-FRET Assay Kit

Item number: BPS-50222-1

The BCL-2 TR-FRET Assay Kit is designed to measure the inhibition of BCL-2 binding to its ligand in a homogeneous 384 reaction format. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and...
Keywords: Bcl, Bcl2, B cell lymphoma 2,
Application: Small molecular inhibitor screening, drug discovery, HTS
Species reactivity: human
From 1,005.00€ *
Review
CD20 Positive Cell Isolation Kit
CD20 Positive Cell Isolation Kit

Item number: BPS-82132-1

The CD20 Positive Cell Isolation Kit is designed to magnetically separate CD20-expressing cells from a complex immune cell population. This kit is optimized for the isolation of CD20 positive cells from normal human peripheral blood mononuclear cells (PBMCs). Cells are incubated with the antibody:bead complex and...
Keywords: Bp35, CD20, MS4A1, B-lymphocyte antigen CD20, B-lymphocyte surface antigen B1, Leukocyte surface antigen Leu-16,...
Application: Isolation, depletion
Species reactivity: human
From 162.00€ *
Review
200x Ubi-Mix(TM)
200x Ubi-Mix(TM)

Item number: BPS-82185-1

The 200x Ubi-Mix(TM) is a mix of Europium cryptate-labeled Ubiquitin (donor), Cy5-labeled Ubiquitin (acceptor), and unlabeled Ubiquitin at a ratio optimized for TR-FRET based assays where long and/or branched polyubiquitin chains are formed. Under conditions where short ubiquitin chains or multi-monoubiquitylation...
Keywords: UBB,
Application: Optimized for use with BPS-78856
Species reactivity: human
From 1,453.00€ *
Review
COX1 Inhibitor Screening Assay Kit
COX1 Inhibitor Screening Assay Kit

Item number: BPS-82203

The COX1 Inhibitor Screening Assay Kit is designed to measure COX1 (cyclooxygenase 1) activity for screening and profiling applications. The assay kit comes in a convenient 96-well format, with enough purified recombinant COX1, Amplex(TM) Red, Arachidonic Diluent, COX Assay Buffer, and 100% ethanol for 100 enzyme...
Keywords: PHS 1, COX-1, PGHS-1, PGH synthase 1, Cyclooxygenase-1, Prostaglandin H2 synthase 1, Prostaglandin G/H synthase 1,...
Application: Enzyme kinetics, small molecule inhibitor screening, drug discovery, HTS
Species reactivity: human
685.00€ *
Review
2-Step DNase I Assay Kit
2-Step DNase I Assay Kit

Item number: BPS-82213

The 2-Step DNase I Assay Kit is a homogeneous fluorescent based assay kit designed to measure DNase I activity for screening and profiling applications. The assay kit comes in a convenient 96-well format, with enough purified recombinant DNase I, DNA substrate, GroovyGreen(TM) dye, assay buffer and co-factors for...
Keywords: Deoxyribonuclease-1, EC:3.1.21.1
Application: Enzyme kinetics, small molecule inhibitor screening, HTS
1,182.00€ *
Review
Chemi-Verse(TM) AKT1 Kinase Assay Kit
Chemi-Verse(TM) AKT1 Kinase Assay Kit

Item number: BPS-82218

The Chemi-Verse(TM) AKT1 Kinase Assay Kit is designed to measure AKT1 (protein kinase B) kinase activity for screening and profiling applications using ADP-Glo(TM) as a detection reagent. The assay kit comes in a convenient 96-well format, with enough purified recombinant AKT1 kinase, kinase substrate, ATP, and...
Keywords: PKB, AKT1, PKB alpha, RAC-PK-alpha, Protein kinase B, Proto-oncogene c-Akt, Protein kinase B alpha, RAC-alpha...
Application: Enzyme kinetics, small molecule inhibitor screening, drug discovery, HTS
Species reactivity: human
842.00€ *
Review
CUL1 Intrachain TR-FRET Assay Kit
CUL1 Intrachain TR-FRET Assay Kit

Item number: BPS-82219

The CUL1 Intrachain TR-FRET Assay Kit is a homogeneous, sensitive TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) assay kit designed to measure CUL1 (cullin 1) auto-ubiquitination. It utilizes a Europium-labeled ubiquitin (Ub) donor as well as Cy5-labeled Ub acceptor to complete the TR-FRET pairing....
Keywords: Cullin-1, CUL1, CUL-1,
Application: Inhibitor screening, drug discovery, HTS, IC determination, real-time kinetic studies
1,285.00€ *
Review
Chemi-Verse(TM) ABL2 Kinase Assay Kit
Chemi-Verse(TM) ABL2 Kinase Assay Kit

Item number: BPS-82220

The Chemi-Verse(TM) ABL2 Kinase Assay Kit is designed to measure ABL2 (Abelson-Related gene protein) kinase activity for screening and profiling applications using ADP-Glo(TM) as a detection reagent. The assay kit comes in a convenient 96-well format, with enough purified recombinant ABL2 kinase (amino acids...
Keywords: ABL2, ABLL, EC=2.7.10.2, Tyrosine-protein kinase ARG, Abelson-related gene protein, Tyrosine-protein kinase ABL2, Abelson...
Application: Enzyme kinetics, small molecule inhibitor screening, drug discovery, HTS
Species reactivity: human
842.00€ *
Review
Chemi-Verse(TM) PAK2 Kinase Assay Kit
Chemi-Verse(TM) PAK2 Kinase Assay Kit

Item number: BPS-82221

The Chemi-Verse(TM) PAK2 Kinase Assay Kit is designed to measure PAK2 (P21 protein (Cdc42/Rac)-activated kinase 2) serine/threonine kinase activity for screening and profiling applications using ADP-Glo(TM) as a detection reagent. The assay kit comes in a convenient 96-well format, with enough purified recombinant...
Keywords: PAK2,
Application: Enzyme kinetics, small molecule inhibitor screening, drug discovery, HTS
Species reactivity: human
908.00€ *
Review
218 from 274 pages