mFluor(TM) Violet 510 goat anti-rabbit IgG (H+L) *Cross-Absorbed*

Molecular weight ~150 kDa. Absorbance (nm): 410. Correction Factor (260 nm) 0.464. Correction Factor (280 nm) 0.366. Extinction coefficient (cm^-1 M^-1): 25000 (PBS). Excitation (nm): 412. Emission (nm): 505. Quantum yield 0.86 (PBS). mFluor(TM) Violet 510 goat anti-rabbit conjugates are secondary antibodies designed for optimal performance in immunoassay applications, including flow cytometry, immunofluorescence, and confocal microscopy. These conjugates consist of goat-derived polyclonal antibodies with high affinity and specificity towards rabbit IgG, conjugated to the bright and stable mFluor(TM) Violet 510 fluorochrome. This conjugation is optimized to ensure minimal non-specific binding and enhanced signal clarity, with rigorous purification steps to remove unconjugated components. Provided in a ready-to-use format with a recommended dilution range, the conjugate undergoes stringent quality control tests for performance and specificity. Its compatibility with a wide range of rabbit primary antibodies and the exceptional contrast provided by mFluor(TM) Violet 510 fluorescence makes it a reliable tool for detecting diverse target antigens in multicolor staining protocols. mFluor(TM) Violet 510 is optimally excited by the violet laser and emits bright green fluorescence maximally at 505 nm. These affinity-purified goat anti-rabbit secondary antibodies are valuable for their versatility and sensitivity, enabling efficient detection, sorting, or purification of specific targets through effective signal amplification in research applications. To minimize cross-reactivity, these goat anti-rabbit IgG whole antibodies have been cross-adsorbed against human, horse, rabbit, and bovine IgG.