mFluor(TM) UV 375 goat anti-rabbit IgG (H+L) *Cross-Absorbed*

Molecular weight ~150 kDa. Absorbance (nm): 342. Correction Factor (260 nm) 0.099. Correction Factor (280 nm) 0.138. Extinction coefficient (cm^-1 M^-1): 30000 (PBS). Excitation (nm): 351. Emission (nm): 387. Quantum yield 0.94 (PBS). mFluor(TM) UV375 goat anti-rabbit conjugates are secondary antibodies designed for optimal performance in immunoassay applications, including flow cytometry, immunofluorescence, and confocal microscopy. These conjugates consist of goat-derived polyclonal antibodies with high affinity and specificity towards rabbit IgG, conjugated to the bright and stable mFluor(TM) UV375 fluorochrome. This conjugation is optimized to ensure minimal non-specific binding and enhanced signal clarity, with rigorous purification steps to remove unconjugated components. Provided in a ready-to-use format with a recommended dilution range, the conjugate undergoes stringent quality control tests for performance and specificity. Its compatibility with a wide range of rabbit primary antibodies and the exceptional contrast provided by mFluor(TM) UV375 fluorescence makes it a reliable tool for detecting diverse target antigens in multicolor staining protocols. mFluor(TM) UV375 is optimally excited by the UV laser and emits maximally at 387 nm. These affinity-purified goat anti-rabbit secondary antibodies are valuable for their versatility and sensitivity, enabling efficient detection, sorting, or purification of specific targets through effective signal amplification in research applications. To minimize cross-reactivity, these goat anti-mouse IgG whole antibodies have been cross-adsorbed against human, horse, mouse, and bovine IgG.