Anti-Sheep IgG HRP conjugated (Sheep TrueBlot®), clone eB270

Anti-Sheep IgG HRP conjugated (Sheep TrueBlot®), clone eB270
Item number Size Datasheet Manual SDS Delivery time Quantity Price
18-8815-31 50 µl - -

6 - 10 business days*

186.00€
 
Sheep IgG TrueBlot® is a unique horseradish peroxidase conjugated anti-sheep IgG immunoblotting... more
Product information "Anti-Sheep IgG HRP conjugated (Sheep TrueBlot®), clone eB270"
Sheep IgG TrueBlot® is a unique horseradish peroxidase conjugated anti-sheep IgG immunoblotting (second step) reagent. Sheep IgG TrueBlot® enables detection of immunoblotted target protein bands, without hindrance by interfering immunoprecipitating immunoglobulin heavy and light chains. It is easy to generate publication-quality IP/Western Blot data with Sheep IgG TrueBlot®, simply substitute the conventional HRP anti-sheep IgG blotting reagent with Sheep IgG TrueBlot® and follow the prescribed protocol for sample preparation and immunoblotting. Sheep IgG TrueBlot® is ideal for use in protocols involving immunoblotting of immunoprecipitated proteins. TrueBlot preferentially detects the non-reduced form of sheep IgG over the reduced, SDS-denatured form of IgG. When the immunoprecipitate is fully reduced immediately prior to SDS-gel electrophoresis, reactivity of Sheep IgG TrueBlot® with the 55 kDa heavy chains and the 23 kDa light chains of the immunoprecipitating antibody is minimized thereby eliminating interference by the heavy and light chains of the immunoprecipitating antibody in IP/Western blotting applications. Applications include studies examining post-translational modification (e.g., phosphorylation or acetylation) or protein-protein interactions. Sheep IgG TrueBlot may also be used for detection in immunoblotting assays that do not employ immunoprecipitation.
Keywords: TrueBlot®, IP/WB
Supplier: Rockland Immunochemicals
Supplier-Nr: 18-8815-31

Properties

Application: WB
Antibody Type: Monoclonal
Clone: eB270
Conjugate: HRP
Host: Mouse
Species reactivity: sheep

Database Information

Handling & Safety

Storage: -20°C
Shipping: +4°C (International: +4°C)
Caution
Our products are for laboratory research use only: Not for administration to humans!
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