Secondary Antibody Fragments

Antibody fragments such as Fab (fragment antigen binding) or F(ab’)2 are obtained by cleavage of whole immunoglobulin G (IgG) proteins with papain or pepsin respectively. Since the entire Fc portion of the antibody is absent, these proteins are smaller and can penetrate tissue more efficiently. Another benefit: less background staining in tissues and cells expressing Fc-receptors. Fragmented secondary antibodies are also frequently used to block free binding sites, when several species-identical primary antibodies are used.

Antibody fragments such as Fab ( fragment antigen binding ) or F(ab’)2 are obtained by cleavage of whole immunoglobulin G (IgG) proteins with papain or pepsin respectively. Since the entire Fc... read more »
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Secondary Antibody Fragments

Antibody fragments such as Fab (fragment antigen binding) or F(ab’)2 are obtained by cleavage of whole immunoglobulin G (IgG) proteins with papain or pepsin respectively. Since the entire Fc portion of the antibody is absent, these proteins are smaller and can penetrate tissue more efficiently. Another benefit: less background staining in tissues and cells expressing Fc-receptors. Fragmented secondary antibodies are also frequently used to block free binding sites, when several species-identical primary antibodies are used.

57 from 58 pages
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Anti-IgG, F2 Fragment
Anti-IgG, F2 Fragment

Item number: I1903-32L.100

Human IgG consists of four subclasses (1-4) that can be recognized by antigen differences in their heavy chains. Each subclass has different biological and physiochemical properties. The IgG subclass may be preferentiallly produced in response to different antigens. The Fab fragment (fragment antigen binding) is the...
Application: ELISA, FC, WB
Host: Mouse
Species reactivity: human
692.00€ *
Review
Anti-IgG F(c) F(ab')2, FITC conjugated
Anti-IgG F(c) F(ab')2, FITC conjugated

Item number: I1903-58.1

Applications: , Suitable for use in FLISA (Fluorescent), Flow Cytometry and Immunofluorescence. Other applications not tested. Recommended Dilution: FLISA (Fluorescent): 1:10,000-:50,000, IF Microscopy: 1:1,000-1:5,000, Flow Cytometry: 1:500-1:2500, Optimal dilutions to be determined by the researcher....
Application: FLISA, FC, IF, WB
Host: Goat
Species reactivity: human
802.00€ *
Review
Anti-IgG, IgA, IgM, H&L, F(ab)'2 Fragment (AP)
Anti-IgG, IgA, IgM, H&L, F(ab)'2 Fragment (AP)

Item number: I1904-26G1.500

Applications: , Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:500-1:2000, Western Blot: 1:500-1:2000 , Immunohistochemistry: 1:20-1:2000, Immunocytochemistry: 1:50-1:5000 , Optimal dilutions to be determined by the...
Keywords: Anti-IgG, IgA, IgM, H&L, F(ab)Õ2 Fragment (AP)
Application: ELISA, ICC, IHC, WB
Host: Goat
Species reactivity: human
404.00€ *
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Anti-IgG F(c) F(ab')2, Rhodamine conjugated
Anti-IgG F(c) F(ab')2, Rhodamine conjugated

Item number: I1904-50P1.1

Applications: , Suitable for use in ELISA and Immunofluorescence as well as other antibody based fluorescent assays. Other applications have not been tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Label: Tetramethylrhodamine isothiocyanate (TRITC) (Molecular Weight 444...
Application: ELISA, IF, WB
Host: Goat
Species reactivity: rat
832.00€ *
Review
Anti-IgG F(c) F(ab')2, X-adsorbed, Texas Red conjugated
Anti-IgG F(c) F(ab')2, X-adsorbed, Texas Red conjugated

Item number: I1904-50Q1.1

Applications:, Suitable for use in FLISA and Immunofluorescence as well as other antibody based fluorescent assays. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Absorption Wavelength: 596nm Emission Wavelength: 620nm, Fluorochrome/Protein Ratio: 2.9...
Application: FLISA, IF, WB
Host: Goat
Species reactivity: rat
897.00€ *
Review
Anti-IgM (mu Chain), F(ab)Õ2 Fragment
Anti-IgM (mu Chain), F(ab)Õ2 Fragment

Item number: I1905-04D.1

Applications: , Suitable for use in ELISA. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12...
Application: ELISA
Host: Goat
Species reactivity: human
485.00€ *
Review
Anti-IgM (mu Chain), F(ab)Õ2 Fragment (AP)
Anti-IgM (mu Chain), F(ab)Õ2 Fragment (AP)

Item number: I1905-04D1.500

Applications: , Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:500-1:2000 , Western Blot: 1:500-1:2000 , Immunohistochemistry: 1:20-1:2000, Immunocytochemistry: 1:50-1:5000, Optimal dilutions to be determined by the...
Application: ELISA, ICC, IHC, WB
Host: Goat
Species reactivity: human
540.00€ *
Review
Anti-IgM (mu Chain), F(ab)Õ2 Fragment (Biotin)
Anti-IgM (mu Chain), F(ab)Õ2 Fragment (Biotin)

Item number: I1905-04D2.500

Applications: , Suitable for use in ELISA. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12...
Application: ELISA
Host: Goat
Species reactivity: human
596.00€ *
Review
Anti-IgM (mu Chain), F(ab)Õ2 Fragment (FITC)
Anti-IgM (mu Chain), F(ab)Õ2 Fragment (FITC)

Item number: I1905-04D4.500

Applications: , Suitable for use in Flow Cytometry, Immunohistochemistry, Immunocytochemistry and Immunomicroscopy. Other applications not tested. Recommended Dilution: Immunohistochemistry: 1:20-1:2000 , Immunocytochemistry: 1:50-1:5000, Optimal dilutions to be determined by the researcher. Absorption: 494nm ,...
Application: FC, ICC, IHC
Host: Goat
Species reactivity: human
517.00€ *
Review
Anti-IgM (mu Chain), F(ab)Õ2 Fragment (HRP)
Anti-IgM (mu Chain), F(ab)Õ2 Fragment (HRP)

Item number: I1905-04D5.500

Applications: , Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:10,000-1:50,000, Western Blot: 1:10,000-1:50,000, Immunohistochemistry: 1:500-1:5000, Optimal dilutions to be determined by the researcher. Storage and Stability:...
Application: ELISA, IHC, WB
Host: Goat
Species reactivity: human
477.00€ *
Review
Anti-IgM (mu Chain), F(ab)Õ2 Fragment, X-Adsorbed
Anti-IgM (mu Chain), F(ab)Õ2 Fragment, X-Adsorbed

Item number: I1905-04E.1

Applications: , Suitable for use in ELISA. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12...
Application: ELISA
Host: Goat
Species reactivity: human
502.00€ *
Review
Anti-IgM (mu Chain), F(ab)Õ2 Fragment, X-Adsorbed (AP)
Anti-IgM (mu Chain), F(ab)Õ2 Fragment, X-Adsorbed (AP)

Item number: I1905-04E1.500

Applications: , Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:500-1:2000 , Western Blot: 1:500-1:2000 , Immunohistochemistry: 1:20-1:2000, Immunocytochemistry: 1:50-1:5000, Optimal dilutions to be determined by...
Application: ELISA, ICC, IHC, WB
Host: Goat
Species reactivity: human
540.00€ *
Review
57 from 58 pages