Secondary Antibody Fragments

Antibody fragments such as Fab (fragment antigen binding) or F(ab’)2 are obtained by cleavage of whole immunoglobulin G (IgG) proteins with papain or pepsin respectively. Since the entire Fc portion of the antibody is absent, these proteins are smaller and can penetrate tissue more efficiently. Another benefit: less background staining in tissues and cells expressing Fc-receptors. Fragmented secondary antibodies are also frequently used to block free binding sites, when several species-identical primary antibodies are used.

Antibody fragments such as Fab ( fragment antigen binding ) or F(ab’)2 are obtained by cleavage of whole immunoglobulin G (IgG) proteins with papain or pepsin respectively. Since the entire Fc... read more »
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Secondary Antibody Fragments

Antibody fragments such as Fab (fragment antigen binding) or F(ab’)2 are obtained by cleavage of whole immunoglobulin G (IgG) proteins with papain or pepsin respectively. Since the entire Fc portion of the antibody is absent, these proteins are smaller and can penetrate tissue more efficiently. Another benefit: less background staining in tissues and cells expressing Fc-receptors. Fragmented secondary antibodies are also frequently used to block free binding sites, when several species-identical primary antibodies are used.

57 from 58 pages
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Anti-IgG F(c) F(ab')2, FITC conjugated
Anti-IgG F(c) F(ab')2, FITC conjugated

Item number: I1903-58.1

Applications: , Suitable for use in FLISA (Fluorescent), Flow Cytometry and Immunofluorescence. Other applications not tested. Recommended Dilution: FLISA (Fluorescent): 1:10,000-:50,000, IF Microscopy: 1:1,000-1:5,000, Flow Cytometry: 1:500-1:2500, Optimal dilutions to be determined by the researcher....
Application: FLISA, FC, IF, WB
Host: Goat
Species reactivity: human
802.00€ *
Review
Anti-IgG, IgA, IgM, H&L, F(ab)'2 Fragment (AP)
Anti-IgG, IgA, IgM, H&L, F(ab)'2 Fragment (AP)

Item number: I1904-26G1.500

Applications: , Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:500-1:2000, Western Blot: 1:500-1:2000 , Immunohistochemistry: 1:20-1:2000, Immunocytochemistry: 1:50-1:5000 , Optimal dilutions to be determined by the...
Keywords: Anti-IgG, IgA, IgM, H&L, F(ab)Õ2 Fragment (AP)
Application: ELISA, ICC, IHC, WB
Host: Goat
Species reactivity: human
557.00€ *
Review
Anti-IgG F(c) F(ab')2, Rhodamine conjugated
Anti-IgG F(c) F(ab')2, Rhodamine conjugated

Item number: I1904-50P1.1

Applications: , Suitable for use in ELISA and Immunofluorescence as well as other antibody based fluorescent assays. Other applications have not been tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Label: Tetramethylrhodamine isothiocyanate (TRITC) (Molecular Weight 444...
Application: ELISA, IF, WB
Host: Goat
Species reactivity: rat
832.00€ *
Review
Anti-IgG F(c) F(ab')2, X-adsorbed, Texas Red conjugated
Anti-IgG F(c) F(ab')2, X-adsorbed, Texas Red conjugated

Item number: I1904-50Q1.1

Applications:, Suitable for use in FLISA and Immunofluorescence as well as other antibody based fluorescent assays. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Absorption Wavelength: 596nm Emission Wavelength: 620nm, Fluorochrome/Protein Ratio: 2.9...
Application: FLISA, IF, WB
Host: Goat
Species reactivity: rat
897.00€ *
Review
Anti-IgM (mu Chain), F(ab)Õ2 Fragment
Anti-IgM (mu Chain), F(ab)Õ2 Fragment

Item number: I1905-04D.1

Applications: , Suitable for use in ELISA. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12...
Application: ELISA
Host: Goat
Species reactivity: human
595.00€ *
Review
Anti-IgM (mu Chain), F(ab)Õ2 Fragment (AP)
Anti-IgM (mu Chain), F(ab)Õ2 Fragment (AP)

Item number: I1905-04D1.500

Applications: , Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:500-1:2000 , Western Blot: 1:500-1:2000 , Immunohistochemistry: 1:20-1:2000, Immunocytochemistry: 1:50-1:5000, Optimal dilutions to be determined by the...
Application: ELISA, ICC, IHC, WB
Host: Goat
Species reactivity: human
746.00€ *
Review
Anti-IgM (mu Chain), F(ab)Õ2 Fragment (Biotin)
Anti-IgM (mu Chain), F(ab)Õ2 Fragment (Biotin)

Item number: I1905-04D2.500

Applications: , Suitable for use in ELISA. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12...
Application: ELISA
Host: Goat
Species reactivity: human
596.00€ *
Review
Anti-IgM (mu Chain), F(ab)Õ2 Fragment (FITC)
Anti-IgM (mu Chain), F(ab)Õ2 Fragment (FITC)

Item number: I1905-04D4.500

Applications: , Suitable for use in Flow Cytometry, Immunohistochemistry, Immunocytochemistry and Immunomicroscopy. Other applications not tested. Recommended Dilution: Immunohistochemistry: 1:20-1:2000 , Immunocytochemistry: 1:50-1:5000, Optimal dilutions to be determined by the researcher. Absorption: 494nm ,...
Application: FC, ICC, IHC
Host: Goat
Species reactivity: human
635.00€ *
Review
Anti-IgM (mu Chain), F(ab)Õ2 Fragment (HRP)
Anti-IgM (mu Chain), F(ab)Õ2 Fragment (HRP)

Item number: I1905-04D5.500

Applications: , Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:10,000-1:50,000, Western Blot: 1:10,000-1:50,000, Immunohistochemistry: 1:500-1:5000, Optimal dilutions to be determined by the researcher. Storage and Stability:...
Application: ELISA, IHC, WB
Host: Goat
Species reactivity: human
557.00€ *
Review
Anti-IgM (mu Chain), F(ab)Õ2 Fragment, X-Adsorbed
Anti-IgM (mu Chain), F(ab)Õ2 Fragment, X-Adsorbed

Item number: I1905-04E.1

Applications: , Suitable for use in ELISA. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12...
Application: ELISA
Host: Goat
Species reactivity: human
502.00€ *
Review
Anti-IgM (mu Chain), F(ab)Õ2 Fragment, X-Adsorbed (AP)
Anti-IgM (mu Chain), F(ab)Õ2 Fragment, X-Adsorbed (AP)

Item number: I1905-04E1.500

Applications: , Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:500-1:2000 , Western Blot: 1:500-1:2000 , Immunohistochemistry: 1:20-1:2000, Immunocytochemistry: 1:50-1:5000, Optimal dilutions to be determined by...
Application: ELISA, ICC, IHC, WB
Host: Goat
Species reactivity: human
746.00€ *
Review
Anti-IgM (mu Chain), F(ab)Õ2 Fragment, X-Adsorbed (Biotin)
Anti-IgM (mu Chain), F(ab)Õ2 Fragment, X-Adsorbed (Biotin)

Item number: I1905-04E2.500

Applications: , Suitable for use in ELISA, Western Blot, Immunohistochemistry and Flow Cytometry. Other applications not tested. Recommended Dilutions: ELISA: 1:20,000-1:500,000, Western Blot: 1:20,000-1:500,000, Immunohistochemistry: 1:500-1:5000, Optimal dilutions to be determined by the researcher. Storage and...
Application: ELISA, FC, IHC, WB
Host: Goat
Species reactivity: human
557.00€ *
Review
57 from 58 pages