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Secondary Antibodies

Secondary antibodies allow the indirect detection of an antigen by binding to the constant region of the primary antibody. To achieve this, the secondary antibody needs to possess a reactivity to the species (e.g. mouse, rabbit or goat) and to the class (e.g. IgG or IgM) of the primary antibody. Secondary antibodies are often labeled with enzymes (e.g. alkaline phosphatases). They convert substrates into coloured products and this process can be analyzed photometrically. Besides the use of enzymes, the labeling of antibodies with fluorescent probes such as FITC or DyLight 488 is a widely used method. 

Secondary antibodies allow the indirect detection of an antigen by binding to the constant region of the primary antibody. To achieve this, the secondary antibody needs to possess a reactivity to... read more »
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Secondary Antibodies

Secondary antibodies allow the indirect detection of an antigen by binding to the constant region of the primary antibody. To achieve this, the secondary antibody needs to possess a reactivity to the species (e.g. mouse, rabbit or goat) and to the class (e.g. IgG or IgM) of the primary antibody. Secondary antibodies are often labeled with enzymes (e.g. alkaline phosphatases). They convert substrates into coloured products and this process can be analyzed photometrically. Besides the use of enzymes, the labeling of antibodies with fluorescent probes such as FITC or DyLight 488 is a widely used method. 

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Anti-Human IgG F(c) [Goat] HRP conjugated
Anti-Human IgG F(c) [Goat] HRP conjugated

Item number: ICT-6291

ICT
Affinity purified, anti-human IgG Fc specific goat IgG was covalently coupled with HRP using a maleimide (M)- facilitated conjugation technique. In this process, free sulfhydryl groups are added to the anti-human IgG Fc prep just prior to its reaction with a 4 fold molar excess of HRP-M. This process leads to an...
Application: ELISA, WB
Host: Goat
Species reactivity: human
204.00€ *
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Anti-Mouse IgG1 cross-adsorbed, HRP conjugated
Anti-Mouse IgG1 cross-adsorbed, HRP conjugated

Item number: A90-205P

Bethyl
Whole IgG
Application: WB, IHC, ICC, ELISA
Host: Goat
Species reactivity: mouse
279.00€ *
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Anti-Goat IgG (H&L) [Donkey] Peroxidase conjugated
Anti-Goat IgG (H&L) [Donkey] Peroxidase conjugated

Item number: 605-703-002

Rockland
Format - Whole IgG
Application: ELISA, IHC, WB
Host: Donkey
Species reactivity: goat
272.00€ *
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Anti-Mouse IgG (H&L) (Goat), ATTO 647N conjugated (Min X Bv Ch Gt GP Ham Hs Hu Rb Rt & Sh Serum Prot
Anti-Mouse IgG (H&L) (Goat), ATTO 647N conjugated (Min X...

Item number: 610-156-121S

Rockland
Format - Whole IgG
Keywords: AT647N, ATTO 647N, ATTO-TEC 647N
Application: FLISA, IFM, WB
Host: Goat
Species reactivity: mouse
From 194.00€ *
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Anti-Rabbit IgG (H&L) [Donkey] Peroxidase conjugated
Anti-Rabbit IgG (H&L) [Donkey] Peroxidase conjugated

Item number: 611-703-127

Rockland
Format - Whole IgG
Application: ELISA, IHC, WB
Host: Donkey
Species reactivity: rabbit
400.00€ *
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Anti-Rabbit IgG (H&L), DyLight(TM) 549 conjugated
Anti-Rabbit IgG (H&L), DyLight(TM) 549 conjugated

Item number: 611-742-127

Rockland
Maximally cross-adsorbed and affinity purified donkey anti-rabbit IgG (H+L) conjugated to DyLight(TM) 549. High fluorescence quantum yield, very high photostability, high specificity with no detectable off target binding.
Keywords: Immunofluorescent Microscopy
Application: FLISA, IFM, WB
Host: Donkey
Species reactivity: Rabbit
177.00€ *
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Anti-Rabbit IgG (H&L) (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins), DyLight 405 conjugate
Anti-Rabbit IgG (H&L) (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt...

Item number: 611-746-127

Rockland
DyLight(TM) dyes are characterized by high fluorescence quantum yield, very high photostability, high specificity with no detectable off target binding.
Keywords: Immunofluorescent Microscopy
Application: FLISA, IFM, WB
Host: Donkey
Species reactivity: rabbit
177.00€ *
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Anti-Rat IgG (H&L) [Goat] Peroxidase conjugated
Anti-Rat IgG (H&L) [Goat] Peroxidase conjugated

Item number: 612-1302

Rockland
Format - Whole IgG
Application: ELISA, IHC, WB
Host: Goat
Species reactivity: rat
220.00€ *
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Anti-MOUSE IgG (H&L), DyLight(TM) 800 Conjugated
Anti-MOUSE IgG (H&L), DyLight(TM) 800 Conjugated

Item number: 610-145-002

Rockland
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose followed by conjugation to fluorochrome and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse...
Application: FLISA, IFM, WB
Host: Goat
Species reactivity: mouse
From 171.00€ *
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Cy7(R) goat anti-rabbit IgG (H+L)
Cy7(R) goat anti-rabbit IgG (H+L)

Item number: ABD-16872

AAT Bioquest
Ex: 749 nm. Em: 776 nm. Soluble in: Water. AAT Bioquest's anti-rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. This Cy7-labeled secondary antibody was prepared...
Application: IF
Host: Goat
Species reactivity: rabbit
218.00€ *
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Anti-Rabbit IgG-heavy and light chain cross-adsorbed, DyLight(R) 488 conjugated
Anti-Rabbit IgG-heavy and light chain cross-adsorbed,...

Item number: A120-208D2

Bethyl
Format - Whole IgG, minimum reactivity to bovine, chicken, goat, human, mouse, pig & rat
Application: WB, IHC, ICC, FC, IF
Host: Donkey
Species reactivity: rabbit
216.00€ *
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Anti-Llama IgG-heavy and light chain, HRP conjugated
Anti-Llama IgG-heavy and light chain, HRP conjugated

Item number: A160-100P

Bethyl
Format - Whole IgG
Application: WB, IHC, ICC, ELISA
Host: Goat
Species reactivity: llama
210.00€ *
Show details
Review
8 from 659 pages
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