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Secondary Antibodies

Secondary antibodies allow the indirect detection of an antigen by binding to the constant region of the primary antibody. To achieve this, the secondary antibody needs to possess a reactivity to the species (e.g. mouse, rabbit or goat) and to the class (e.g. IgG or IgM) of the primary antibody. Secondary antibodies are often labeled with enzymes (e.g. alkaline phosphatases). They convert substrates into coloured products and this process can be analyzed photometrically. Besides the use of enzymes, the labeling of antibodies with fluorescent probes such as FITC or DyLight 488 is a widely used method. 

Secondary antibodies allow the indirect detection of an antigen by binding to the constant region of the primary antibody. To achieve this, the secondary antibody needs to possess a reactivity to... read more »
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Secondary Antibodies

Secondary antibodies allow the indirect detection of an antigen by binding to the constant region of the primary antibody. To achieve this, the secondary antibody needs to possess a reactivity to the species (e.g. mouse, rabbit or goat) and to the class (e.g. IgG or IgM) of the primary antibody. Secondary antibodies are often labeled with enzymes (e.g. alkaline phosphatases). They convert substrates into coloured products and this process can be analyzed photometrically. Besides the use of enzymes, the labeling of antibodies with fluorescent probes such as FITC or DyLight 488 is a widely used method. 

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658 from 658 pages
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CF(R)740 Goat Anti-Rabbit IgG (H+L), Highly Cross-Adsorbed, 2 mg/mL
CF(R)740 Goat Anti-Rabbit IgG (H+L), Highly...

Item number: BOT-20990-250uL

Biotium
This product is prepared by labeling highly cross-adsorbed goat anti-rabbit IgG (H+L) with CF(R)740 dye. CF(R)740 dye has excitation/emission at 740/775 nm. To minimize cross-reactivity, the antibody has been adsorbed against human, mouse, and rat serum. Concentration: 2 mg/mL Storage buffer: PBS, 50% glycerol, 2...
Keywords: CF(R)740 Goat Anti-Rabbit IgG (H+L), Highly Cross-Adsorbed, 2 mg/mL,
Application: IF, IHC (paraffin), IHC (frozen), WB
Host: Goat
Species reactivity: rabbit
From 211.00€ *
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CF(R)740 Donkey Anti-Mouse IgG (H+L), Highly Cross-Adsorbed, 2 mg/mL
CF(R)740 Donkey Anti-Mouse IgG (H+L), Highly...

Item number: BOT-20991-250uL

Biotium
This product is prepared by labeling highly cross-adsorbed donkey anti-mouse IgG (H+L) with CF(R)740 dye. To minimize cross-reactivity, the antibody has been adsorbed against bovine, chicken, goat, guinea pig, Syrian hamster, horse, human, rabbit, and sheep serum. Concentration: 2 mg/mL Storage buffer: PBS, 50%...
Keywords: CF(R)740 Donkey Anti-Mouse IgG (H+L), Highly Cross-Adsorbed, 2 mg/mL,
Application: IF, IHC (paraffin), IHC (frozen), WB
Host: Donkey
Species reactivity: mouse
From 201.00€ *
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CF(R)498 Donkey Anti-Goat IgG (H+L), Highly Cross-Adsorbed, single label for STORM 1 mg/mL
CF(R)498 Donkey Anti-Goat IgG (H+L), Highly...

Item number: BOT-20992-500uL

Biotium
This product is prepared by labeling highly cross-adsorbed donkey anti-goat IgG (H+L) with CF(R)498 dye and has excitation/emission at 497/525 nm. Single label antibodies have an average of one dye molecule per antibody molecule, which is reported to be optimal for STORM imaging. Concentration: 1 mg/mL Storage...
Keywords: CF(R)498 Donkey Anti-Goat IgG (H+L), Highly Cross-Adsorbed, single label for STORM 1 mg/mL,
Application: STORM
Host: Donkey
Species reactivity: goat
From 92.00€ *
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CF(R)498 Donkey Anti-Guinea Pig IgG (H+L), Highly Cross-Adsorbed, single label for STORM, 1 mg/mL
CF(R)498 Donkey Anti-Guinea Pig IgG (H+L), Highly...

Item number: BOT-20993-500uL

Biotium
Highly cross-adsorbed donkey anti-guinea pig IgG (H+L) labeled with CF(R)498 dye. Single CF(R) dye label (DOL=1), optimized for super-resolution imaging by STORM. Adsorbed against bovine, chicken, goat, Syrian hamster, horse, human, mouse, rabbit, sheep and rat serum. Concentration: 1 mg/mL Storage buffer: PBS, 50%...
Keywords: CF(R)498 Donkey Anti-Guinea Pig IgG (H+L), Highly Cross-Adsorbed, single label for STORM, 1 mg/mL,
Application: STORM
Host: Donkey
Species reactivity: guinea pig
From 95.00€ *
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CF(R)498 Donkey Anti-Mouse IgG (H+L), Highly Cross-Adsorbed, single label for STORM, 1 mg/mL
CF(R)498 Donkey Anti-Mouse IgG (H+L), Highly...

Item number: BOT-20994-500uL

Biotium
This product is prepared by labeling highly cross-adsorbed donkey anti-mouse IgG (H+L) with CF(R)498 dye and has excitation/emission at 497/525 nm. Single label antibodies have an average of one dye molecule per antibody molecule, which is reported to be optimal for STORM imaging. Concentration: 1 mg/mL Storage...
Keywords: CF(R)498 Donkey Anti-Mouse IgG (H+L), Highly Cross-Adsorbed, single label for STORM, 1 mg/mL,
Application: STORM
Host: Donkey
Species reactivity: mouse
From 92.00€ *
Show details
Review
CF(R)498 Donkey Anti-Rabbit IgG (H+L), Highly Cross-Adsorbed, single label for STORM, 1 mg/mL
CF(R)498 Donkey Anti-Rabbit IgG (H+L), Highly...

Item number: BOT-20995-500uL

Biotium
This product is prepared by labeling highly cross-adsorbed donkey anti-mouse IgG (H+L) with CF(R)498 dye and has excitation/emission at 497/525 nm. Single label antibodies have an average of one dye molecule per antibody molecule, which is reported to be optimal for STORM imaging. Concentration: 1 mg/mL Storage...
Keywords: CF(R)498 Donkey Anti-Rabbit IgG (H+L), Highly Cross-Adsorbed, single label for STORM, 1 mg/mL,
Application: STORM
Host: Donkey
Species reactivity: rabbit
From 92.00€ *
Show details
Review
CF(R)498 Goat Anti-Mouse IgG (H+L), Highly Cross-Adsorbed, single label for STORM, 1 mg/mL
CF(R)498 Goat Anti-Mouse IgG (H+L), Highly...

Item number: BOT-20996-500uL

Biotium
This product is prepared by labeling highly cross-adsorbed goat anti-mouse IgG (H+L) with CF(R)498 dye and has excitation/emission at 497/525 nm. Single label antibodies have an average of one dye molecule per antibody molecule, which is reported to be optimal for STORM imaging. Concentration: 1 mg/mL Storage...
Keywords: CF(R)498 Goat Anti-Mouse IgG (H+L), Highly Cross-Adsorbed, single label for STORM, 1 mg/mL,
Application: STORM
Host: Goat
Species reactivity: mouse
From 92.00€ *
Show details
Review
CF(R)498 Goat Anti-Rabbit IgG (H+L), Highly Cross-Adsorbed, single label for STORM, 1 mg/mL
CF(R)498 Goat Anti-Rabbit IgG (H+L), Highly...

Item number: BOT-20997-500uL

Biotium
This product is prepared by labeling highly cross-adsorbed goat anti-rabbit IgG (H+L) with CF(R)498 dye and has excitation/emission at 497/525 nm. Single label antibodies have an average of one dye molecule per antibody molecule, which is reported to be optimal for STORM imaging. Concentration: 1 mg/mL Storage...
Keywords: CF(R)498 Goat Anti-Rabbit IgG (H+L), Highly Cross-Adsorbed, single label for STORM, 1 mg/mL,
Application: STORM
Host: Goat
Species reactivity: rabbit
From 92.00€ *
Show details
Review
658 from 658 pages
This website uses cookies, which are necessary for the technical operation of the website and are always set. Other cookies, which increase the usability of this website, serve for direct advertising or simplify interaction with other websites and social networks, will only be used with your consent. More information
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