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Secondary Antibodies

Secondary antibodies allow the indirect detection of an antigen by binding to the constant region of the primary antibody. To achieve this, the secondary antibody needs to possess a reactivity to the species (e.g. mouse, rabbit or goat) and to the class (e.g. IgG or IgM) of the primary antibody. Secondary antibodies are often labeled with enzymes (e.g. alkaline phosphatases). They convert substrates into coloured products and this process can be analyzed photometrically. Besides the use of enzymes, the labeling of antibodies with fluorescent probes such as FITC or DyLight 488 is a widely used method. 

Secondary antibodies allow the indirect detection of an antigen by binding to the constant region of the primary antibody. To achieve this, the secondary antibody needs to possess a reactivity to... read more »
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Secondary Antibodies

Secondary antibodies allow the indirect detection of an antigen by binding to the constant region of the primary antibody. To achieve this, the secondary antibody needs to possess a reactivity to the species (e.g. mouse, rabbit or goat) and to the class (e.g. IgG or IgM) of the primary antibody. Secondary antibodies are often labeled with enzymes (e.g. alkaline phosphatases). They convert substrates into coloured products and this process can be analyzed photometrically. Besides the use of enzymes, the labeling of antibodies with fluorescent probes such as FITC or DyLight 488 is a widely used method. 

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657 from 658 pages
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CF(R)505 F(ab')2 fragment of Goat Anti-Rabbit IgG (H+L), 2 mg/mL
CF(R)505 F(ab')2 fragment of Goat Anti-Rabbit IgG (H+L),...

Item number: BOT-20915-250uL

Biotium
This product is prepared by labeling high quality F(ab')2 fragment of goat anti-rabbit IgG (H+L) with CF(R)505 dye. CF(R) dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Concentration: 2 mg/mL...
Keywords: CF(R)505 F(ab')2 fragment of Goat Anti-Rabbit IgG (H+L), 2 mg/mL,
Application: IF, IHC (paraffin), IHC (frozen), WB
Host: Goat
Species reactivity: rabbit
From 143.00€ *
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CF(R)505 F(ab')2 fragment of Goat Anti-Mouse IgG (H+L), 2 mg/mL
CF(R)505 F(ab')2 fragment of Goat Anti-Mouse IgG (H+L), 2...

Item number: BOT-20916-250uL

Biotium
This product is prepared by labeling high quality F(ab')2 fragment of goat anti-mouse IgG (H+L) with CF(R)505 dye. CF(R) dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Concentration: 2 mg/mL...
Keywords: CF(R)505 F(ab')2 fragment of Goat Anti-Mouse IgG (H+L), 2 mg/mL,
Application: IF, IHC (paraffin), IHC (frozen), WB
Host: Goat
Species reactivity: mouse
From 143.00€ *
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Review
CF(R)660C F(ab')2 fragment of Goat Anti-Rabbit IgG (H+L), 2 mg/mL
CF(R)660C F(ab')2 fragment of Goat Anti-Rabbit IgG (H+L),...

Item number: BOT-20917-250uL

Biotium
This product is prepared by labeling high quality F(ab')2 fragment of goat anti-rabbit IgG (H+L) with CF(R)660C dye. CF(R) dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Concentration: 2 mg/mL...
Keywords: CF(R)660C F(ab')2 fragment of Goat Anti-Rabbit IgG (H+L), 2 mg/mL,
Application: IF, IHC (paraffin), IHC (frozen), WB
Host: Goat
Species reactivity: rabbit
From 143.00€ *
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CF(R)660C F(ab')2 fragment of Goat Anti-Mouse IgG (H+L), 2 mg/mL
CF(R)660C F(ab')2 fragment of Goat Anti-Mouse IgG (H+L),...

Item number: BOT-20918-250uL

Biotium
This product is prepared by labeling high quality F(ab')2 fragment of goat anti-mouse IgG (H+L) with CF(R)660C dye. CF(R) dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Concentration: 2 mg/mL...
Keywords: CF(R)660C F(ab')2 fragment of Goat Anti-Mouse IgG (H+L), 2 mg/mL,
Application: IF, IHC (paraffin), IHC (frozen), WB
Host: Goat
Species reactivity: mouse
From 143.00€ *
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Review
CF(R)750 F(ab')2 fragment of Goat Anti-Rabbit IgG (H+L), 2 mg/mL
CF(R)750 F(ab')2 fragment of Goat Anti-Rabbit IgG (H+L),...

Item number: BOT-20919-250uL

Biotium
This product is prepared by labeling high quality F(ab')2 fragment of goat anti-rabbit IgG (H+L) with CF(R)750 dye. CF(R) dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Concentration: 2 mg/mL...
Keywords: CF(R)750 F(ab')2 fragment of Goat Anti-Rabbit IgG (H+L), 2 mg/mL,
Application: IF, IHC (paraffin), IHC (frozen), WB
Host: Goat
Species reactivity: rabbit
From 143.00€ *
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Review
CF(R)750 F(ab')2 fragment of Goat Anti-Mouse IgG (H+L), 2 mg/mL
CF(R)750 F(ab')2 fragment of Goat Anti-Mouse IgG (H+L), 2...

Item number: BOT-20920-250uL

Biotium
This product is prepared by labeling high quality F(ab')2 fragment of goat anti-mouse IgG (H+L) with CF(R)750 dye. CF(R) dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Concentration: 2 mg/mL...
Keywords: CF(R)750 F(ab')2 fragment of Goat Anti-Mouse IgG (H+L), 2 mg/mL,
Application: IF, IHC (paraffin), IHC (frozen), WB
Host: Goat
Species reactivity: mouse
From 143.00€ *
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CF(R)740 Goat Anti-Mouse IgG (H+L), 2 mg/mL
CF(R)740 Goat Anti-Mouse IgG (H+L), 2 mg/mL

Item number: BOT-20984-500uL

Biotium
This product is prepared by labeling high quality goat anti-mouse IgG (H+L) with CF(R)740 dye. CF(R)740 dye has excitation/emission at 740/775 nm. Concentration: 2 mg/mL Storage buffer: PBS, 50% glycerol, 2 mg/ml BSA, 0.05% azide
Keywords: CF(R)740 Goat Anti-Mouse IgG (H+L), 2 mg/mL,
Application: IF, IHC (paraffin), IHC (frozen), WB
Host: Goat
Species reactivity: mouse
From 189.00€ *
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CF(R)740 Goat Anti-Rabbit IgG (H+L), 2 mg/mL
CF(R)740 Goat Anti-Rabbit IgG (H+L), 2 mg/mL

Item number: BOT-20985-500uL

Biotium
This product is prepared by labeling high quality goat anti-rabbit IgG (H+L) with CF(R)740 dye. CF(R)740 dye has excitation/emission at 740/775 nm. Concentration: 2 mg/mL Storage buffer: PBS, 50% glycerol, 2 mg/ml BSA, 0.05% azide
Keywords: CF(R)740 Goat Anti-Rabbit IgG (H+L), 2 mg/mL,
Application: IF, IHC (paraffin), IHC (frozen), WB
Host: Goat
Species reactivity: rabbit
From 189.00€ *
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CF(R)740 Donkey Anti-Rabbit IgG (H+L), Highly Cross-Adsorbed, 2 mg/mL
CF(R)740 Donkey Anti-Rabbit IgG (H+L), Highly...

Item number: BOT-20986-250uL

Biotium
This product is prepared by labeling highly cross-adsorbed donkey anti-rabbit IgG (H+L) with CF(R)740 dye. To minimize cross-reactivity, the antibody has been adsorbed against bovine, chicken, goat, guinea pig, Syrian hamster, horse, human, mouse, rat, and sheep serum. Concentration: 2 mg/mL Storage buffer: PBS, 50%...
Keywords: CF(R)740 Donkey Anti-Rabbit IgG (H+L), Highly Cross-Adsorbed, 2 mg/mL,
Application: IF, IHC (paraffin), IHC (frozen), WB
Host: Donkey
Species reactivity: rabbit
From 201.00€ *
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CF(R)740 Donkey Anti-Goat IgG (H+L), highly Crossed-Adsorbed, 2 mg/mL
CF(R)740 Donkey Anti-Goat IgG (H+L), highly...

Item number: BOT-20987-250uL

Biotium
This product is prepared by labeling highly cross-adsorbed donkey anti-goat IgG (H+L) with CF(R)740 dye. To minimize cross-reactivity, the antibody has been adsorbed against chicken, guinea pig, Syrian hamster, horse, human, mouse, rabbit, and rat serum. Concentration: 2 mg/mL Storage buffer: PBS, 50% glycerol, 2...
Keywords: CF(R)740 Donkey Anti-Goat IgG (H+L), highly Crossed-Adsorbed, 2 mg/mL,
Application: IF, IHC (paraffin), IHC (frozen), WB
Host: Donkey
Species reactivity: goat
From 201.00€ *
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CF(R)740 Goat Anti-Mouse IgG (H+L), Highly Cross-Adsorbed, 2 mg/mL
CF(R)740 Goat Anti-Mouse IgG (H+L), Highly...

Item number: BOT-20988-250uL

Biotium
This product is prepared by labeling highly cross-adsorbed goat anti-mouse IgG (H+L) with CF(R)740 dye. To minimize cross-reactivity, the antibody has been adsorbed against human, bovine, horse, rabbit, and swine serum. Concentration: 2 mg/mL Storage buffer: PBS, 50% glycerol, 2 mg/ml BSA, 0.05% azide
Keywords: CF(R)740 Goat Anti-Mouse IgG (H+L), Highly Cross-Adsorbed, 2 mg/mL,
Application: IF, IHC (paraffin), IHC (frozen), WB
Host: Goat
Species reactivity: mouse
From 211.00€ *
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CF(R)740 Donkey Anti-Rat, Highly Cross-Adsorbed, 2mg/mL
CF(R)740 Donkey Anti-Rat, Highly Cross-Adsorbed, 2mg/mL

Item number: BOT-20989-250uL

Biotium
This product is prepared by labeling highly cross-adsorbed donkey anti-rat IgG (H+L) with CF(R)740 dye. To minimize cross-reactivity, the antibody has been adsorbed against bovine, chicken, goat, guinea pig, Syrian hamster, horse, human, mouse, rabbit, and sheep serum proteins. Concentration: 2 mg/mL Storage buffer:...
Keywords: CF(R)740 Donkey Anti-Rat, Highly Cross-Adsorbed, 2mg/mL,
Application: IF, IHC (paraffin), IHC (frozen), WB
Host: Donkey
Species reactivity: rat
From 196.00€ *
Show details
Review
657 from 658 pages
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