Secondary Antibodies

Secondary antibodies allow the indirect detection of an antigen by binding to the constant region of the primary antibody. To achieve this, the secondary antibody needs to possess a reactivity to the species (e.g. mouse, rabbit or goat) and to the class (e.g. IgG or IgM) of the primary antibody. Secondary antibodies are often labeled with enzymes (e.g. alkaline phosphatases). They convert substrates into coloured products and this process can be analyzed photometrically. Besides the use of enzymes, the labeling of antibodies with fluorescent probes such as FITC or DyLight 488 is a widely used method. 

Secondary antibodies allow the indirect detection of an antigen by binding to the constant region of the primary antibody. To achieve this, the secondary antibody needs to possess a reactivity to... read more »
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Secondary Antibodies

Secondary antibodies allow the indirect detection of an antigen by binding to the constant region of the primary antibody. To achieve this, the secondary antibody needs to possess a reactivity to the species (e.g. mouse, rabbit or goat) and to the class (e.g. IgG or IgM) of the primary antibody. Secondary antibodies are often labeled with enzymes (e.g. alkaline phosphatases). They convert substrates into coloured products and this process can be analyzed photometrically. Besides the use of enzymes, the labeling of antibodies with fluorescent probes such as FITC or DyLight 488 is a widely used method. 

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CF(R)583R Llama Anti-Mouse IgG (H&L), 2mg/mL
CF(R)583R Llama Anti-Mouse IgG (H&L), 2mg/mL

Item number: BOT-20908-500uL

This product is prepared by labeling an affinity-purified llama anti-mouse IgG with CF(R)583R dye. CF(R) dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Concentration: 2 mg/mL Storage buffer: PBS,...
Keywords: CF(R)583R Llama Anti-Mouse IgG (H&L), 2mg/mL,
Application: IF, IHC (paraffin), IHC (frozen), WB
Host: Llama
Species reactivity: mouse
From 122.00€ *
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CF(R)583R Llama Anti-Rabbit IgG (H&L), 2mg/mL
CF(R)583R Llama Anti-Rabbit IgG (H&L), 2mg/mL

Item number: BOT-20909-500uL

This product is prepared by labeling an affinity-purified llama anti-rabbit IgG with CF(R)583R dye. CF(R) dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Concentration: 2 mg/mL Storage buffer:...
Keywords: CF(R)583R Llama Anti-Rabbit IgG (H&L), 2mg/mL,
Application: IF, IHC (paraffin), IHC (frozen), WB
Host: Llama
Species reactivity: rabbit
From 122.00€ *
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CF(R)583R Rabbit Anti-Mouse IgG (H+L), Highly Cross-Adsorbed, 2 mg/mL
CF(R)583R Rabbit Anti-Mouse IgG (H+L), Highly...

Item number: BOT-20911-500uL

This product is prepared by labeling highly cross-adsorbed rabbit anti-mouse IgG (H+L) with CF(R)583R dye. CF(R) dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Concentration: 2 mg/mL Storage...
Keywords: CF(R)583R Rabbit Anti-Mouse IgG (H+L), Highly Cross-Adsorbed, 2 mg/mL,
Application: IF, IHC (paraffin), IHC (frozen), WB
Host: Rabbit
Species reactivity: mouse
From 149.00€ *
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CF(R)583R Rabbit Anti-Rat IgG (H+L), Highly Cross-Adsorbed, 2 mg/mL
CF(R)583R Rabbit Anti-Rat IgG (H+L), Highly...

Item number: BOT-20912-500uL

This product is prepared by labeling highly cross-adsorbed rabbit anti-rat IgG (H+L) with CF(R)583R dye. CF(R) dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Concentration: 2 mg/mL Storage...
Keywords: CF(R)583R Rabbit Anti-Rat IgG (H+L), Highly Cross-Adsorbed, 2 mg/mL,
Application: IF, IHC (paraffin), IHC (frozen), WB
Host: Rabbit
Species reactivity: rat
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CF(R)583R F(ab')2 fragment of Goat Anti-Rabbit IgG (H+L), 2 mg/mL
CF(R)583R F(ab')2 fragment of Goat Anti-Rabbit IgG (H+L),...

Item number: BOT-20913-250uL

This product is prepared by labeling high quality F(ab')2 fragment of goat anti-rabbit IgG (H+L) with CF(R)583R dye. CF(R) dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Concentration: 2 mg/mL...
Keywords: CF(R)583R F(ab')2 fragment of Goat Anti-Rabbit IgG (H+L), 2 mg/mL,
Application: IF, IHC (paraffin), IHC (frozen), WB
Host: Goat
Species reactivity: rabbit
From 143.00€ *
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CF(R)583R F(ab')2 fragment of Goat Anti-Mouse IgG (H+L), 2 mg/mL
CF(R)583R F(ab')2 fragment of Goat Anti-Mouse IgG (H+L),...

Item number: BOT-20914-250uL

This product is prepared by labeling high quality F(ab')2 fragment of goat anti-mouse IgG (H+L) with CF(R)583R dye. CF(R) dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Concentration: 2 mg/mL...
Keywords: CF(R)583R F(ab')2 fragment of Goat Anti-Mouse IgG (H+L), 2 mg/mL,
Application: IF, IHC (paraffin), IHC (frozen), WB
Host: Goat
Species reactivity: mouse
From 143.00€ *
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CF(R)505 F(ab')2 fragment of Goat Anti-Rabbit IgG (H+L), 2 mg/mL
CF(R)505 F(ab')2 fragment of Goat Anti-Rabbit IgG (H+L),...

Item number: BOT-20915-250uL

This product is prepared by labeling high quality F(ab')2 fragment of goat anti-rabbit IgG (H+L) with CF(R)505 dye. CF(R) dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Concentration: 2 mg/mL...
Keywords: CF(R)505 F(ab')2 fragment of Goat Anti-Rabbit IgG (H+L), 2 mg/mL,
Application: IF, IHC (paraffin), IHC (frozen), WB
Host: Goat
Species reactivity: rabbit
From 143.00€ *
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CF(R)505 F(ab')2 fragment of Goat Anti-Mouse IgG (H+L), 2 mg/mL
CF(R)505 F(ab')2 fragment of Goat Anti-Mouse IgG (H+L), 2...

Item number: BOT-20916-250uL

This product is prepared by labeling high quality F(ab')2 fragment of goat anti-mouse IgG (H+L) with CF(R)505 dye. CF(R) dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Concentration: 2 mg/mL...
Keywords: CF(R)505 F(ab')2 fragment of Goat Anti-Mouse IgG (H+L), 2 mg/mL,
Application: IF, IHC (paraffin), IHC (frozen), WB
Host: Goat
Species reactivity: mouse
From 143.00€ *
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CF(R)660C F(ab')2 fragment of Goat Anti-Rabbit IgG (H+L), 2 mg/mL
CF(R)660C F(ab')2 fragment of Goat Anti-Rabbit IgG (H+L),...

Item number: BOT-20917-250uL

This product is prepared by labeling high quality F(ab')2 fragment of goat anti-rabbit IgG (H+L) with CF(R)660C dye. CF(R) dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Concentration: 2 mg/mL...
Keywords: CF(R)660C F(ab')2 fragment of Goat Anti-Rabbit IgG (H+L), 2 mg/mL,
Application: IF, IHC (paraffin), IHC (frozen), WB
Host: Goat
Species reactivity: rabbit
From 143.00€ *
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CF(R)660C F(ab')2 fragment of Goat Anti-Mouse IgG (H+L), 2 mg/mL
CF(R)660C F(ab')2 fragment of Goat Anti-Mouse IgG (H+L),...

Item number: BOT-20918-250uL

This product is prepared by labeling high quality F(ab')2 fragment of goat anti-mouse IgG (H+L) with CF(R)660C dye. CF(R) dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Concentration: 2 mg/mL...
Keywords: CF(R)660C F(ab')2 fragment of Goat Anti-Mouse IgG (H+L), 2 mg/mL,
Application: IF, IHC (paraffin), IHC (frozen), WB
Host: Goat
Species reactivity: mouse
From 143.00€ *
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CF(R)750 F(ab')2 fragment of Goat Anti-Mouse IgG (H+L), 2 mg/mL
CF(R)750 F(ab')2 fragment of Goat Anti-Mouse IgG (H+L), 2...

Item number: BOT-20920-250uL

This product is prepared by labeling high quality F(ab')2 fragment of goat anti-mouse IgG (H+L) with CF(R)750 dye. CF(R) dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Concentration: 2 mg/mL...
Keywords: CF(R)750 F(ab')2 fragment of Goat Anti-Mouse IgG (H+L), 2 mg/mL,
Application: IF, IHC (paraffin), IHC (frozen), WB
Host: Goat
Species reactivity: mouse
From 143.00€ *
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CF(R)740 Goat Anti-Mouse IgG (H+L), 2 mg/mL
CF(R)740 Goat Anti-Mouse IgG (H+L), 2 mg/mL

Item number: BOT-20984-500uL

This product is prepared by labeling high quality goat anti-mouse IgG (H+L) with CF(R)740 dye. CF(R)740 dye has excitation/emission at 740/775 nm. Concentration: 2 mg/mL Storage buffer: PBS, 50% glycerol, 2 mg/ml BSA, 0.05% azide
Keywords: CF(R)740 Goat Anti-Mouse IgG (H+L), 2 mg/mL,
Application: IF, IHC (paraffin), IHC (frozen), WB
Host: Goat
Species reactivity: mouse
From 189.00€ *
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656 from 658 pages