Secondary Antibodies

Secondary antibodies allow the indirect detection of an antigen by binding to the constant region of the primary antibody. To achieve this, the secondary antibody needs to possess a reactivity to the species (e.g. mouse, rabbit or goat) and to the class (e.g. IgG or IgM) of the primary antibody. Secondary antibodies are often labeled with enzymes (e.g. alkaline phosphatases). They convert substrates into coloured products and this process can be analyzed photometrically. Besides the use of enzymes, the labeling of antibodies with fluorescent probes such as FITC or DyLight 488 is a widely used method. 

Secondary antibodies allow the indirect detection of an antigen by binding to the constant region of the primary antibody. To achieve this, the secondary antibody needs to possess a reactivity to... read more »
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Secondary Antibodies

Secondary antibodies allow the indirect detection of an antigen by binding to the constant region of the primary antibody. To achieve this, the secondary antibody needs to possess a reactivity to the species (e.g. mouse, rabbit or goat) and to the class (e.g. IgG or IgM) of the primary antibody. Secondary antibodies are often labeled with enzymes (e.g. alkaline phosphatases). They convert substrates into coloured products and this process can be analyzed photometrically. Besides the use of enzymes, the labeling of antibodies with fluorescent probes such as FITC or DyLight 488 is a widely used method. 

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CF(R)583R Rabbit Anti-Goat IgG (H+L), 2 mg/mL
CF(R)583R Rabbit Anti-Goat IgG (H+L), 2 mg/mL

Item number: BOT-20910-500uL

This product is prepared by labeling high quality rabbit anti-goat IgG (H+L) with CF(R)583R dye. CF(R) dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Concentration: 2 mg/mL Storage buffer: PBS,...
Keywords: CF(R)583R Rabbit Anti-Goat IgG (H+L), 2 mg/mL,
Application: IF, IHC (paraffin), IHC (frozen), WB
Host: Rabbit
Species reactivity: goat
From 119.00€ *
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CF(R)750 F(ab')2 fragment of Goat Anti-Rabbit IgG (H+L), 2 mg/mL
CF(R)750 F(ab')2 fragment of Goat Anti-Rabbit IgG (H+L),...

Item number: BOT-20919-250uL

This product is prepared by labeling high quality F(ab')2 fragment of goat anti-rabbit IgG (H+L) with CF(R)750 dye. CF(R) dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Concentration: 2 mg/mL...
Keywords: CF(R)750 F(ab')2 fragment of Goat Anti-Rabbit IgG (H+L), 2 mg/mL,
Application: IF, IHC (paraffin), IHC (frozen), WB
Host: Goat
Species reactivity: rabbit
From 143.00€ *
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CF(R)740 Donkey Anti-Goat IgG (H+L), highly Crossed-Adsorbed, 2 mg/mL
CF(R)740 Donkey Anti-Goat IgG (H+L), highly...

Item number: BOT-20987-250uL

This product is prepared by labeling highly cross-adsorbed donkey anti-goat IgG (H+L) with CF(R)740 dye. To minimize cross-reactivity, the antibody has been adsorbed against chicken, guinea pig, Syrian hamster, horse, human, mouse, rabbit, and rat serum. Concentration: 2 mg/mL Storage buffer: PBS, 50% glycerol, 2...
Keywords: CF(R)740 Donkey Anti-Goat IgG (H+L), highly Crossed-Adsorbed, 2 mg/mL,
Application: IF, IHC (paraffin), IHC (frozen), WB
Host: Donkey
Species reactivity: goat
From 201.00€ *
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CF(R)498 Donkey Anti-Mouse IgG (H+L), Highly Cross-Adsorbed, single label for STORM, 1 mg/mL
CF(R)498 Donkey Anti-Mouse IgG (H+L), Highly...

Item number: BOT-20994-500uL

This product is prepared by labeling highly cross-adsorbed donkey anti-mouse IgG (H+L) with CF(R)498 dye and has excitation/emission at 497/525 nm. Single label antibodies have an average of one dye molecule per antibody molecule, which is reported to be optimal for STORM imaging. Concentration: 1 mg/mL Storage...
Keywords: CF(R)498 Donkey Anti-Mouse IgG (H+L), Highly Cross-Adsorbed, single label for STORM, 1 mg/mL,
Application: STORM
Host: Donkey
Species reactivity: mouse
From 92.00€ *
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CF(R)498 Goat Anti-Mouse IgG (H+L), Highly Cross-Adsorbed, single label for STORM, 1 mg/mL
CF(R)498 Goat Anti-Mouse IgG (H+L), Highly...

Item number: BOT-20996-500uL

This product is prepared by labeling highly cross-adsorbed goat anti-mouse IgG (H+L) with CF(R)498 dye and has excitation/emission at 497/525 nm. Single label antibodies have an average of one dye molecule per antibody molecule, which is reported to be optimal for STORM imaging. Concentration: 1 mg/mL Storage...
Keywords: CF(R)498 Goat Anti-Mouse IgG (H+L), Highly Cross-Adsorbed, single label for STORM, 1 mg/mL,
Application: STORM
Host: Goat
Species reactivity: mouse
From 92.00€ *
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CF(R)583R Donkey Anti-Goat IgG (H+L), Highly Cross-Adsorbed, 2 mg/mL
CF(R)583R Donkey Anti-Goat IgG (H+L), Highly...

Item number: BOT-20892-500uL

This product is prepared by labeling highly cross-adsorbed donkey anti-goat IgG (H+L) with CF(R)583R dye. To minimize cross-reactivity, the antibody has been adsorbed against chicken, guinea pig, Syrian hamster, horse, human, mouse, rabbit, and rat serum. Concentration: 2 mg/mL Storage buffer: PBS, 50% glycerol, 2...
Keywords: CF(R)583R Donkey Anti-Goat IgG (H+L), Highly Cross-Adsorbed, 2 mg/mL,
Application: IF, IHC (paraffin), IHC (frozen), WB
Host: Donkey
Species reactivity: goat
From 143.00€ *
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CF(R)583R Donkey Anti-Rabbit IgG (H+L), Highly Cross-Adsorbed, 2 mg/mL
CF(R)583R Donkey Anti-Rabbit IgG (H+L), Highly...

Item number: BOT-20895-500uL

This product is prepared by labeling highly cross-adsorbed donkey anti-rabbit IgG (H+L) with CF(R)583R dye. To minimize cross-reactivity, the antibody has been adsorbed against bovine, chicken, goat, guinea pig, Syrian hamster, horse, human, mouse, rat, and sheep serum. Concentration: 2 mg/mL Storage buffer: PBS,...
Keywords: CF(R)583R Donkey Anti-Rabbit IgG (H+L), Highly Cross-Adsorbed, 2 mg/mL,
Application: IF, IHC (paraffin), IHC (frozen), WB
Host: Donkey
Species reactivity: rabbit
From 143.00€ *
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CF(R)583R Goat Anti-Guinea Pig IgG (H+L), 2 mg/mL
CF(R)583R Goat Anti-Guinea Pig IgG (H+L), 2 mg/mL

Item number: BOT-20899-500uL

This product is prepared by labeling high quality goat anti-guinea pig IgG (H+L) with CF(R)583R dye. CF(R) dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Concentration: 2 mg/mL Storage buffer:...
Keywords: CF(R)583R Goat Anti-Guinea Pig IgG (H+L), 2 mg/mL,
Application: IF, IHC (paraffin), IHC (frozen), WB
Host: Goat
Species reactivity: guinea pig
From 115.00€ *
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CF(R)583R Goat Anti-Human IgG (H+L), Highly Cross-Adsorbed, 2 mg/mL
CF(R)583R Goat Anti-Human IgG (H+L), Highly...

Item number: BOT-20901-500uL

This product is prepared by labeling highly cross-adsorbed goat anti-human IgG (H+L) with CF(R)583R dye. To minimize cross-reactivity, the antibody has been adsorbed against bovine, horse, and mouse serum. Concentration: 2 mg/mL Storage buffer: PBS, 50% glycerol, 2 mg/ml BSA, 0.05% azide
Keywords: CF(R)583R Goat Anti-Human IgG (H+L), Highly Cross-Adsorbed, 2 mg/mL,
Application: IF, IHC (paraffin), IHC (frozen), WB
Host: Goat
Species reactivity: human
From 149.00€ *
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CF(R)583R Goat Anti-Rabbit IgG (H+L), Highly Cross-Adsorbed, 2 mg/mL
CF(R)583R Goat Anti-Rabbit IgG (H+L), Highly...

Item number: BOT-20905-500uL

This product is prepared by labeling highly cross-adsorbed goat anti-rabbit IgG (H+L) with CF(R)583R dye. To minimize cross-reactivity, the antibody has been adsorbed against human, mouse, and rat serum. Concentration: 2 mg/mL Storage buffer: PBS, 50% glycerol, 2 mg/ml BSA, 0.05% azide
Keywords: CF(R)583R Goat Anti-Rabbit IgG (H+L), Highly Cross-Adsorbed, 2 mg/mL,
Application: IF, IHC (paraffin), IHC (frozen), WB
Host: Goat
Species reactivity: rabbit
From 143.00€ *
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CF(R)583R Goat Anti-Rat IgG (H+L), Highly Cross-Adsorbed, 2 mg/mL
CF(R)583R Goat Anti-Rat IgG (H+L), Highly Cross-Adsorbed,...

Item number: BOT-20906-500uL

This product is prepared by labeling highly cross-adsorbed goat anti-rat IgG (H+L) with CF(R)583R dye. To minimize cross-reactivity, the antibody has been adsorbed against human, bovine, horse, and rabbit serum. Concentration: 2 mg/mL Storage buffer: PBS, 50% glycerol, 2 mg/ml BSA, 0.05% azide
Keywords: CF(R)583R Goat Anti-Rat IgG (H+L), Highly Cross-Adsorbed, 2 mg/mL,
Application: IF, IHC (paraffin), IHC (frozen), WB
Host: Goat
Species reactivity: rat
From 149.00€ *
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CF(R)583R Goat Anti-Swine IgG (H+L), 2 mg/mL
CF(R)583R Goat Anti-Swine IgG (H+L), 2 mg/mL

Item number: BOT-20907-500uL

This product is prepared by labeling high quality goat anti-swine IgG (H+L) with CF(R)583R dye. CF(R) dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Concentration: 2 mg/mL Storage buffer: PBS,...
Keywords: CF(R)583R Goat Anti-Swine IgG (H+L), 2 mg/mL,
Application: IF, IHC (paraffin), IHC (frozen), WB
Host: Goat
Species reactivity: swine
From 119.00€ *
Review
655 from 658 pages