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Secondary Antibodies

Secondary antibodies allow the indirect detection of an antigen by binding to the constant region of the primary antibody. To achieve this, the secondary antibody needs to possess a reactivity to the species (e.g. mouse, rabbit or goat) and to the class (e.g. IgG or IgM) of the primary antibody. Secondary antibodies are often labeled with enzymes (e.g. alkaline phosphatases). They convert substrates into coloured products and this process can be analyzed photometrically. Besides the use of enzymes, the labeling of antibodies with fluorescent probes such as FITC or DyLight 488 is a widely used method. 

Secondary antibodies allow the indirect detection of an antigen by binding to the constant region of the primary antibody. To achieve this, the secondary antibody needs to possess a reactivity to... read more »
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Secondary Antibodies

Secondary antibodies allow the indirect detection of an antigen by binding to the constant region of the primary antibody. To achieve this, the secondary antibody needs to possess a reactivity to the species (e.g. mouse, rabbit or goat) and to the class (e.g. IgG or IgM) of the primary antibody. Secondary antibodies are often labeled with enzymes (e.g. alkaline phosphatases). They convert substrates into coloured products and this process can be analyzed photometrically. Besides the use of enzymes, the labeling of antibodies with fluorescent probes such as FITC or DyLight 488 is a widely used method. 

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654 from 658 pages
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CF(R)583R Bovine Anti-Goat IgG (H+L), Highly Cross-Adsorbed, 2 mg/mL
CF(R)583R Bovine Anti-Goat IgG (H+L), Highly...

Item number: BOT-20888-500uL

Biotium
This product is bovine anti-goat IgG labeled with CF(R)583R dye. The antibody is created by immunizing a cow host with goat IgG to minimize the reactivity for bovine IgG. It has been adsorbed against bovine, chicken, guinea pig, Syrian hamster, horse, human, mouse, rabbit and rat serum proteins. Concentration: 2...
Keywords: CF(R)583R Bovine Anti-Goat IgG (H+L), Highly Cross-Adsorbed, 2 mg/mL,
Application: IF, IHC (paraffin), IHC (frozen), WB
Host: Bovine
Species reactivity: goat
From 149.00€ *
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CF(R)583R Chicken Anti-Goat IgG (H+L), 2 mg/mL
CF(R)583R Chicken Anti-Goat IgG (H+L), 2 mg/mL

Item number: BOT-20889-500uL

Biotium
This product is prepared by labeling an affinity-purified chicken anti-goat IgG (H+L) with CF(R)583R dye. CF(R) dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Concentration: 2 mg/mL Storage...
Keywords: CF(R)583R Chicken Anti-Goat IgG (H+L), 2 mg/mL,
Application: IF, IHC (paraffin), IHC (frozen), WB
Host: Chicken
Species reactivity: goat
From 119.00€ *
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CF(R)583R Chicken Anti-Mouse IgG (H+L), 2 mg/mL
CF(R)583R Chicken Anti-Mouse IgG (H+L), 2 mg/mL

Item number: BOT-20890-500uL

Biotium
This product is prepared by labeling an affinity-purified chicken anti-mouse IgG (H+L) with CF(R)583R dye. CF(R) dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Concentration: 2 mg/mL Storage...
Keywords: CF(R)583R Chicken Anti-Mouse IgG (H+L), 2 mg/mL,
Application: IF, IHC (paraffin), IHC (frozen), WB
Host: Chicken
Species reactivity: mouse
From 115.00€ *
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CF(R)583R Chicken Anti-Rabbit IgG (H+L), 2 mg/mL
CF(R)583R Chicken Anti-Rabbit IgG (H+L), 2 mg/mL

Item number: BOT-20891-500uL

Biotium
This product is prepared by labeling an affinity-purified chicken anti-rabbit IgG (H+L) with CF(R)583R dye. CF(R) dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Concentration: 2 mg/mL Storage...
Keywords: CF(R)583R Chicken Anti-Rabbit IgG (H+L), 2 mg/mL,
Application: IF, IHC (paraffin), IHC (frozen), WB
Host: Chicken
Species reactivity: rabbit
From 115.00€ *
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CF(R)583R Donkey Anti-Goat IgG (H+L), Highly Cross-Adsorbed, 2 mg/mL
CF(R)583R Donkey Anti-Goat IgG (H+L), Highly...

Item number: BOT-20892-500uL

Biotium
This product is prepared by labeling highly cross-adsorbed donkey anti-goat IgG (H+L) with CF(R)583R dye. To minimize cross-reactivity, the antibody has been adsorbed against chicken, guinea pig, Syrian hamster, horse, human, mouse, rabbit, and rat serum. Concentration: 2 mg/mL Storage buffer: PBS, 50% glycerol, 2...
Keywords: CF(R)583R Donkey Anti-Goat IgG (H+L), Highly Cross-Adsorbed, 2 mg/mL,
Application: IF, IHC (paraffin), IHC (frozen), WB
Host: Donkey
Species reactivity: goat
From 143.00€ *
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CF(R)583R Donkey Anti-Guinea Pig IgG (H+L), Highly Cross-Adsorbed, 2 mg/mL
CF(R)583R Donkey Anti-Guinea Pig IgG (H+L), Highly...

Item number: BOT-20893-500uL

Biotium
This product is prepared by labeling highly cross-adsorbed donkey anti-guinea pig IgG (H+L) with CF(R)583R dye. To minimize cross-reactivity, the antibody has been adsorbed against bovine, chicken, goat, Syrian hamster, horse, human, mouse, rabbit, sheep and rat serum. Concentration: 2 mg/mL Storage buffer: PBS, 50%...
Keywords: CF(R)583R Donkey Anti-Guinea Pig IgG (H+L), Highly Cross-Adsorbed, 2 mg/mL,
Application: IF, IHC (paraffin), IHC (frozen), WB
Host: Donkey
Species reactivity: guinea pig
From 149.00€ *
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CF(R)583R Donkey Anti-Mouse IgG (H+L), Highly Cross-Adsorbed, 2 mg/mL
CF(R)583R Donkey Anti-Mouse IgG (H+L), Highly...

Item number: BOT-20894-500uL

Biotium
This product is prepared by labeling highly cross-adsorbed donkey anti-mouse IgG (H+L) with CF(R)583R dye. To minimize cross-reactivity, the antibody has been adsorbed against bovine, chicken, goat, guinea pig, Syrian hamster, horse, human, rabbit, and sheep serum. Concentration: 2 mg/mL Storage buffer: PBS, 50%...
Keywords: CF(R)583R Donkey Anti-Mouse IgG (H+L), Highly Cross-Adsorbed, 2 mg/mL,
Application: IF, IHC (paraffin), IHC (frozen), WB
Host: Donkey
Species reactivity: mouse
From 143.00€ *
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Review
CF(R)583R Donkey Anti-Rabbit IgG (H+L), Highly Cross-Adsorbed, 2 mg/mL
CF(R)583R Donkey Anti-Rabbit IgG (H+L), Highly...

Item number: BOT-20895-500uL

Biotium
This product is prepared by labeling highly cross-adsorbed donkey anti-rabbit IgG (H+L) with CF(R)583R dye. To minimize cross-reactivity, the antibody has been adsorbed against bovine, chicken, goat, guinea pig, Syrian hamster, horse, human, mouse, rat, and sheep serum. Concentration: 2 mg/mL Storage buffer: PBS,...
Keywords: CF(R)583R Donkey Anti-Rabbit IgG (H+L), Highly Cross-Adsorbed, 2 mg/mL,
Application: IF, IHC (paraffin), IHC (frozen), WB
Host: Donkey
Species reactivity: rabbit
From 143.00€ *
Show details
Review
CF(R)583R Donkey Anti-Rat IgG (H+L), Highly Cross-Adsorbed, 2 mg/mL
CF(R)583R Donkey Anti-Rat IgG (H+L), Highly...

Item number: BOT-20896-500uL

Biotium
This product is prepared by labeling highly cross-adsorbed donkey anti-rat IgG (H+L) with CF(R)583R dye. To minimize cross-reactivity, the antibody has been adsorbed against bovine, chicken, goat, guinea pig, Syrian hamster, horse, human, mouse, rabbit, and sheep serum proteins. Concentration: 2 mg/mL Storage...
Keywords: CF(R)583R Donkey Anti-Rat IgG (H+L), Highly Cross-Adsorbed, 2 mg/mL,
Application: IF, IHC (paraffin), IHC (frozen), WB
Host: Donkey
Species reactivity: rat
From 143.00€ *
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Review
CF(R)583R Donkey Anti-Sheep IgG (H+L), Highly Cross-Adsorbed, 2 mg/mL
CF(R)583R Donkey Anti-Sheep IgG (H+L), Highly...

Item number: BOT-20897-500uL

Biotium
This product is prepared by labeling highly cross-adsorbed donkey anti-sheep IgG (H+L) with CF(R)583R dye. To minimize cross-reactivity, the antibody has been adsorbed against chicken, guinea pig, Syrian hamster, horse, human, mouse, rabbit, and rat serum. Concentration: 2 mg/mL Storage buffer: PBS, 50% glycerol, 2...
Keywords: CF(R)583R Donkey Anti-Sheep IgG (H+L), Highly Cross-Adsorbed, 2 mg/mL,
Application: IF, IHC (paraffin), IHC (frozen), WB
Host: Donkey
Species reactivity: sheep
From 143.00€ *
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CF(R)583R Goat Anti-Chicken IgY (IgG) (H+L), Highly Cross-Adsorbed, 2 mg/mL
CF(R)583R Goat Anti-Chicken IgY (IgG) (H+L), Highly...

Item number: BOT-20898-500uL

Biotium
This product is prepared by labeling highly cross-adsorbed goat anti-chicken IgY (H+L) with CF(R)583R dye. To minimize cross-reactivity, the antibody has been adsorbed against bovine, goat, guinea pig, Syrian hamster, horse, human, mouse, rabbit, rat, and sheep serum. Concentration: 2 mg/mL Storage buffer: PBS, 50%...
Keywords: CF(R)583R Goat Anti-Chicken IgY (IgG) (H+L), Highly Cross-Adsorbed, 2 mg/mL,
Application: IF, IHC (paraffin), IHC (frozen), WB
Host: Goat
Species reactivity: chicken
From 149.00€ *
Show details
Review
CF(R)583R Goat Anti-Guinea Pig IgG (H+L), 2 mg/mL
CF(R)583R Goat Anti-Guinea Pig IgG (H+L), 2 mg/mL

Item number: BOT-20899-500uL

Biotium
This product is prepared by labeling high quality goat anti-guinea pig IgG (H+L) with CF(R)583R dye. CF(R) dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Concentration: 2 mg/mL Storage buffer:...
Keywords: CF(R)583R Goat Anti-Guinea Pig IgG (H+L), 2 mg/mL,
Application: IF, IHC (paraffin), IHC (frozen), WB
Host: Goat
Species reactivity: guinea pig
From 115.00€ *
Show details
Review
654 from 658 pages
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