Secondary Antibodies

Secondary antibodies allow the indirect detection of an antigen by binding to the constant region of the primary antibody. To achieve this, the secondary antibody needs to possess a reactivity to the species (e.g. mouse, rabbit or goat) and to the class (e.g. IgG or IgM) of the primary antibody. Secondary antibodies are often labeled with enzymes (e.g. alkaline phosphatases). They convert substrates into coloured products and this process can be analyzed photometrically. Besides the use of enzymes, the labeling of antibodies with fluorescent probes such as FITC or DyLight 488 is a widely used method. 

Secondary antibodies allow the indirect detection of an antigen by binding to the constant region of the primary antibody. To achieve this, the secondary antibody needs to possess a reactivity to... read more »
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Secondary Antibodies

Secondary antibodies allow the indirect detection of an antigen by binding to the constant region of the primary antibody. To achieve this, the secondary antibody needs to possess a reactivity to the species (e.g. mouse, rabbit or goat) and to the class (e.g. IgG or IgM) of the primary antibody. Secondary antibodies are often labeled with enzymes (e.g. alkaline phosphatases). They convert substrates into coloured products and this process can be analyzed photometrically. Besides the use of enzymes, the labeling of antibodies with fluorescent probes such as FITC or DyLight 488 is a widely used method. 

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Anti-rabbit IgG(H+L) [Goat], Affinipure, HRP-conjugated
Anti-rabbit IgG(H+L) [Goat], Affinipure, HRP-conjugated

Item number: CSB-PA001501GA01RB.100

Host Species: Goat. Isotype: IgG. Buffer: 0.01M Sodium phosphate, 0.25M NaCl, 50% glycerol, pH7.6. Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. Purification Method: The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads. Antigen...
Keywords: HRP-conjugated Affinipure Goat Anti-Rabbit IgG(H+L)
Application: ELISA, WB
Host: Goat
Species reactivity: rabbit
From 79.00€ *
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Anti-Rabbit IgG(H+L) [Goat], Cy3-conjugated
Anti-Rabbit IgG(H+L) [Goat], Cy3-conjugated

Item number: CSB-PA956124GA01Rb.100

Host Species: Goat. Isotype: IgG. Buffer: 0.01M Sodium phosphate, 0.25M NaCl, 50% glycerol, pH7.6. Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. Purification Method: The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads. Antigen...
Keywords: Goat Anti-Rabbit IgG(H+L) Antibody, Cy3-conjugated
Application: IF, FC
Host: Goat
Species reactivity: rabbit
From 79.00€ *
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Anti-IgG (H&L) X-adsorbed, Biotin conjugated
Anti-IgG (H&L) X-adsorbed, Biotin conjugated

Item number: I1903-04Z.1

Applications: , Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:10,000-1:50,000, Western Blot: 1:1000-1:5000, Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately...
Application: ELISA, IHC, WB
Host: Goat
Species reactivity: hamster
802.00€ *
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Anti-IgG F(ab')2, Peroxidase conjugated
Anti-IgG F(ab')2, Peroxidase conjugated

Item number: I1903-21V.1500

Applications: , Suitable for ELISA, Western Blot and Immunohistochemistry. Other applications have not been tested. Recommended Dilutions: ELISA: 1:10,000-1:50,000, Western Blot: 1:1000-1:5000, Immunohistochemistry: 1:500-1:2500, Optimal dilutions to be determined by the researcher. Storage and Stability: Store...
Application: ELISA, IHC, WB
Host: Rabbit
Species reactivity: horse
794.00€ *
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Anti-IgM, Biotin conjugated
Anti-IgM, Biotin conjugated

Item number: I1905-01B.2

Applications: , Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: ELISA: 1:10,000-1:50,000, Immunohistochemistry: 1:1000-1:5000, Western Blot: 1:2000-1:10,000, Optimal dilutions to be determined by the researcher. Storage and Stability: Store...
Application: ELISA, IHC, WB
Host: Goat
Species reactivity: mouse
787.00€ *
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Anti-IgA, Rhodamine conjugated
Anti-IgA, Rhodamine conjugated

Item number: I1890-05T.1

Applications: , Suitable for use in ELISA and Immunofluorescence. Other applications not tested. Recommended Dilution: ELISA: 1:10,000-1:50,000, Immunofluorescence: 1:1000-1:5000, Optimal dilutions to be determined by the researcher. Absorption Wavelength: 550nm Emission Wavelength: 570nm, Fluorochrome/Protein...
Application: ELISA, IF, WB
Host: Rabbit
Species reactivity: human
698.00€ *
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Anti-IgG (H&L) Peroxidase conjugated
Anti-IgG (H&L) Peroxidase conjugated

Item number: I1903-01T.2

Applications:, Suitable for use in ELISA and Immunohistochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:10,000-1:50,000, Immunohistochemistry: 1:500-1:2500, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to...
Application: ELISA, IHC
Host: Rabbit
Species reactivity: bovine
598.00€ *
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Anti-IgG F(ab')2, Peroxidase conjugated
Anti-IgG F(ab')2, Peroxidase conjugated

Item number: I1903-04N.2

Applications: , Suitable for use in ELISA, Western Blot, Immunoelectrophoresis and Immunohistochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:4000-1:20,000., Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term...
Application: ELISA, IHC, WB
Host: Rabbit
Species reactivity: guinea pig
699.00€ *
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Anti-IgG (H&L) X-adsorbed, Alkaline Phosphatase conjugated
Anti-IgG (H&L) X-adsorbed, Alkaline Phosphatase conjugated

Item number: I1903-06A.1

Applications: , Suitable for use in ELISA, Western Blot, Immunohistochemistry. Other applications not tested., , Recommended Dilution: , ELISA: 1:2000-1:10,000, Immunohistochemistry: 1:500-1:2500, Western Blot: 1:500-1:2500, Optimal dilutions to be determined by the researcher. Storage and Stability: Store product...
Application: ELISA, IHC, WB
Host: Goat
Species reactivity: hamster
895.00€ *
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Anti-IgG (H&L) X-adsorbed, Cy5 conjugated
Anti-IgG (H&L) X-adsorbed, Cy5 conjugated

Item number: I1903-12.1

Applications: , Suitable for use in FLISA and Immunofluorescence. Other applications have not been tested. Recommended Dilution: Immunomicroscopy: 1:100-1:1000, Optimal dilutions to be determined by the researcher. Label: Cy5.29 (Cyanine 5.29-OSu). Molecular Weight: 975D. Absorption Wavelength: 650nm, Emission...
Application: FLISA, IF, WB
Host: Goat
Species reactivity: mouse
816.00€ *
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Anti-IgG F(ab')2, X-adsorbed, Phycoerythrin conjugated
Anti-IgG F(ab')2, X-adsorbed, Phycoerythrin conjugated

Item number: I1903-13E.1

Applications: , Suitable for use in Immunomicroscopy, Flow Cytometry, FLISA and Immunofluorescence. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:100-1:250 , Immunofluorescence: 1:100-1:250, Optimal dilutions to be determined by the researcher. Excitation Wavelength: 488 , Emission...
Application: FLISA, FC, IF, WB
Host: Goat
Species reactivity: rat
947.00€ *
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Anti-IgG (H&L) X-adsorbed, FITC conjugated
Anti-IgG (H&L) X-adsorbed, FITC conjugated

Item number: I1903-16F.1

Applications: , Suitable for use in Immunofluorescence and FLISA. Other applications not tested. Recommended Dilution: Immunofluorescence: 1:500-1:2500, Optimal dilutions to be determined by the researcher, Absorption Wavelength: 495 nm , Emission Wavelength: 528 nm, Fluorochrome/Protein Ratio: 2.6 moles FITC per...
Application: FLISA, IF, WB
Host: Goat
Species reactivity: chicken
795.00€ *
Review
652 from 659 pages