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Secondary Antibodies

Secondary antibodies allow the indirect detection of an antigen by binding to the constant region of the primary antibody. To achieve this, the secondary antibody needs to possess a reactivity to the species (e.g. mouse, rabbit or goat) and to the class (e.g. IgG or IgM) of the primary antibody. Secondary antibodies are often labeled with enzymes (e.g. alkaline phosphatases). They convert substrates into coloured products and this process can be analyzed photometrically. Besides the use of enzymes, the labeling of antibodies with fluorescent probes such as FITC or DyLight 488 is a widely used method. 

Secondary antibodies allow the indirect detection of an antigen by binding to the constant region of the primary antibody. To achieve this, the secondary antibody needs to possess a reactivity to... read more »
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Secondary Antibodies

Secondary antibodies allow the indirect detection of an antigen by binding to the constant region of the primary antibody. To achieve this, the secondary antibody needs to possess a reactivity to the species (e.g. mouse, rabbit or goat) and to the class (e.g. IgG or IgM) of the primary antibody. Secondary antibodies are often labeled with enzymes (e.g. alkaline phosphatases). They convert substrates into coloured products and this process can be analyzed photometrically. Besides the use of enzymes, the labeling of antibodies with fluorescent probes such as FITC or DyLight 488 is a widely used method. 

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651 from 659 pages
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Anti-IgE (human) Purified, clone BE5 (Monoclonal) [Mouse]
Anti-IgE (human) Purified, clone BE5 (Monoclonal) [Mouse]

Item number: MON-MON23252

MONOSAN
Immunoglobulin E (IgE) is a 180 kDa soluble protein serving as an antigen-specific unit of mast cell effector mechanisms. IgE has the lowest serum concentration of all immunoglobulins (approximately 0.5 mg/l) in healthy individuals, but upon allergen challenge its concentration in blood increases dramatically....
Application: FC, ELISA
Host: Mouse
Species reactivity: human
218.00€ *
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Review
Anti-IgG (human) (Fab) Purified, clone 4A11 (Monoclonal) [Mouse]
Anti-IgG (human) (Fab) Purified, clone 4A11 (Monoclonal)...

Item number: MON-MON23253

MONOSAN
Immunoglobulin G (IgG) is a 150 kDa soluble protein that serves as a major effector molecule of the humoral immune response in man. Its concentration in blood plasma of healthy individuals is approximately 10 g/l, which accounts for about 75% of the total plasma immunoglobulins. IgG has the highest stability of...
Application: FC, WB, ELISA
Host: Mouse
Species reactivity: human
218.00€ *
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Review
Anti-IgG (human) (Fc) Purified, clone EM-07 (Monoclonal) [Mouse]
Anti-IgG (human) (Fc) Purified, clone EM-07 (Monoclonal)...

Item number: MON-MON23254

MONOSAN
Immunoglobulin G (IgG) is a 150 kDa soluble protein that serves as a major effector molecule of the humoral immune response in man. Its concentration in blood plasma of healthy individuals is approximately 10 g/l, which accounts for about 75% of the total plasma immunoglobulins. IgG has the highest stability of...
Application: WB, ICC, ELISA, FC
Host: Mouse
Species reactivity: human
218.00€ *
Show details
Review
Anti-IgM (human) Purified, clone CH2 (Monoclonal) [Mouse]
Anti-IgM (human) Purified, clone CH2 (Monoclonal) [Mouse]

Item number: MON-MON23255

MONOSAN
Immunoglobulin M (IgM) is produced as a 900 kDa pentamer, which is an efficient complement binder. This antibody type is produced initially in the immune response and it is the first immunoglobulin class to be synthesized by a fetus or newborn. IgM antibodies do not cross the placenta. IgM concentration in blood is...
Application: WB, ICC, ELISA, FC
Host: Mouse
Species reactivity: human
218.00€ *
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Review
Anti-IgG (human) (Fc) FITC, clone EM-07 (Monoclonal) [Mouse]
Anti-IgG (human) (Fc) FITC, clone EM-07 (Monoclonal) [Mouse]

Item number: MON-MON23321F

MONOSAN
Immunoglobulin G (IgG) is a 150 kDa soluble protein that serves as a major effector molecule of the humoral immune response in man. Its concentration in blood plasma of healthy individuals is approximately 10 g/l, which accounts for about 75% of the total plasma immunoglobulins. IgG has the highest stability of...
Application: FC
Host: Mouse
Species reactivity: human
326.00€ *
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Review
Anti-IgG (human) (Fc) HRP, clone EM-07 (Monoclonal) [Mouse]
Anti-IgG (human) (Fc) HRP, clone EM-07 (Monoclonal) [Mouse]

Item number: MON-MON23321H

MONOSAN
Immunoglobulin G (IgG) is a 150 kDa soluble protein that serves as a major effector molecule of the humoral immune response in man. Its concentration in blood plasma of healthy individuals is approximately 10 g/l, which accounts for about 75% of the total plasma immunoglobulins. IgG has the highest stability of...
Application: WB, ELISA
Host: Mouse
Species reactivity: human
412.00€ *
Show details
Review
Anti-IgM (human) FITC, clone CH2 (Monoclonal) [Mouse]
Anti-IgM (human) FITC, clone CH2 (Monoclonal) [Mouse]

Item number: MON-MON23322F

MONOSAN
Immunoglobulin M (IgM) is produced as a 900 kDa pentamer, which is an efficient complement binder. This antibody type is produced initially in the immune response and it is the first immunoglobulin class to be synthesized by a fetus or newborn. IgM antibodies do not cross the placenta. IgM concentration in blood is...
Application: FC
Host: Mouse
Species reactivity: human
326.00€ *
Show details
Review
Anti-IgM (human) HRP, clone CH2 (Monoclonal) [Mouse]
Anti-IgM (human) HRP, clone CH2 (Monoclonal) [Mouse]

Item number: MON-MON23322H

MONOSAN
Immunoglobulin M (IgM) is produced as a 900 kDa pentamer, which is an efficient complement binder. This antibody type is produced initially in the immune response and it is the first immunoglobulin class to be synthesized by a fetus or newborn. IgM antibodies do not cross the placenta. IgM concentration in blood is...
Application: WB, ELISA
Host: Mouse
Species reactivity: human
412.00€ *
Show details
Review
Anti-IgG/A/M (H/L) (mouse) [Goat]
Anti-IgG/A/M (H/L) (mouse) [Goat]

Item number: MON-PS23001

MONOSAN
Goat anti Mouse IgG/A/M reacts with the heavy and light chains of all major classes of mouse immunoglobulin.The antibody has been adsorbed against human serum to minimise cross-reactivity with human immunoglobulins. Storage: Store at -20oC only.Storage in frost-free freezers is not recommended.This product should be...
Application: ELISA, IB, FC
Host: Goat
Species reactivity: mouse
351.00€ *
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Anti-IgG/A/M (H/L):FITC (mouse) [Goat]
Anti-IgG/A/M (H/L):FITC (mouse) [Goat]

Item number: MON-PS23002

MONOSAN
Goat anti Mouse IgG/A/M reacts with the heavy and light chains of all major classes of mouse immunoglobulin.The antibody has been adsorbed against human serum to minimise cross-reactivity with human immunoglobulins. Storage: This product is shipped at ambient temperature. It is recommended to aliquot and store at...
Application: FC
Host: Goat
Species reactivity: mouse
351.00€ *
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Anti-IgG/A/M:FITC (human) [Goat]
Anti-IgG/A/M:FITC (human) [Goat]

Item number: MON-PS23015

MONOSAN
This product is shipped at ambient temperature. Prior to reconstitution store at +4°C.After reconstitution it is recommended to aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature...
Application: ELISA, FC
Host: Goat
Species reactivity: human
585.00€ *
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Anti-rabbit IgG(H+L) [Goat], Affinipure, HRP-conjugated
Anti-rabbit IgG(H+L) [Goat], Affinipure, HRP-conjugated

Item number: CSB-PA001501GA01RB.100

Cusabio
Host Species: Goat. Isotype: IgG. Buffer: 0.01M Sodium phosphate, 0.25M NaCl, 50% glycerol, pH7.6. Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. Purification Method: The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads. Antigen...
Keywords: HRP-conjugated Affinipure Goat Anti-Rabbit IgG(H+L)
Application: ELISA, WB
Host: Goat
Species reactivity: rabbit
From 79.00€ *
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651 from 659 pages
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