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Secondary Antibodies

Secondary antibodies allow the indirect detection of an antigen by binding to the constant region of the primary antibody. To achieve this, the secondary antibody needs to possess a reactivity to the species (e.g. mouse, rabbit or goat) and to the class (e.g. IgG or IgM) of the primary antibody. Secondary antibodies are often labeled with enzymes (e.g. alkaline phosphatases). They convert substrates into coloured products and this process can be analyzed photometrically. Besides the use of enzymes, the labeling of antibodies with fluorescent probes such as FITC or DyLight 488 is a widely used method. 

Secondary antibodies allow the indirect detection of an antigen by binding to the constant region of the primary antibody. To achieve this, the secondary antibody needs to possess a reactivity to... read more »
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Secondary Antibodies

Secondary antibodies allow the indirect detection of an antigen by binding to the constant region of the primary antibody. To achieve this, the secondary antibody needs to possess a reactivity to the species (e.g. mouse, rabbit or goat) and to the class (e.g. IgG or IgM) of the primary antibody. Secondary antibodies are often labeled with enzymes (e.g. alkaline phosphatases). They convert substrates into coloured products and this process can be analyzed photometrically. Besides the use of enzymes, the labeling of antibodies with fluorescent probes such as FITC or DyLight 488 is a widely used method. 

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650 from 659 pages
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Anti-mouse IgG Fab [Rabbit]
Anti-mouse IgG Fab [Rabbit]

Item number: CSB-PA00590E0Rb.500

Cusabio
Host Species: Rabbit. Isotype: IgG. Buffer: Preservative: 0.03% Proclin 300Constituents: 50% Glycerol, 0.01M PBS, PH 7.4. Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. Purification Method: Caprylic Acid Ammonium Sulfate Precipitation purified. Antigen Species: Mouse
Keywords: Rabbit anti-Mouse IgG Fab Antibody
Application: ELISA
Host: Rabbit
Species reactivity: mouse
170.00€ *
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Anti-mouse IgG(H+L) [Goat], FITC conjugated
Anti-mouse IgG(H+L) [Goat], FITC conjugated

Item number: CSB-PA980279.300

Cusabio
Host Species: Goat. Isotype: IgG. Buffer: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. Purification Method: The antibody was affinity-purified from goat antiserum by affinity-chromatography using specific immunogen....
Keywords: Goat Anti-Mouse IgG(H+L) Antibody,FITC conjugated
Application: IHC, IF, FC
Host: Goat
Species reactivity: mouse
109.00€ *
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Anti-mouse IgG(H+L) [Goat], HRP conjugated
Anti-mouse IgG(H+L) [Goat], HRP conjugated

Item number: CSB-PA644737.300

Cusabio
Host Species: Goat. Isotype: IgG. Buffer: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. Purification Method: The antibody was affinity-purified from goat antiserum by affinity-chromatography using specific immunogen....
Keywords: Goat Anti-Mouse IgG(H+L) Antibody,HRP conjugated
Application: ELISA, WB, IHC, IF, IP, FC
Host: Goat
Species reactivity: mouse
109.00€ *
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Anti-human IgG [Goat], Fcgamma fragment specific
Anti-human IgG [Goat], Fcgamma fragment specific

Item number: CSB-PA793692.100

Cusabio
Host Species: Goat. Isotype: IgG. Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6. Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. Purification Method: The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads. Antigen Species: Human
Keywords: Goat Anti-Human IgG, Fcgamma fragment specific Antibody
Application: ELISA
Host: Goat
Species reactivity: human
From 38.00€ *
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Anti-IgG (H&L) Peroxidase conjugated
Anti-IgG (H&L) Peroxidase conjugated

Item number: I1903-04E.2

USBiological
Applications: , Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications have not been tested. Recommended Dilution: ELISA: 1:4000-1:20,000, Assayed against 1.0ug of guinea pig IgG in a standard capture ELISA using ABTS as a substrate for 30 minutes at RT., Western Blot: 1:2000-1:10,000,...
Application: ELISA, IHC, WB
Host: Rabbit
Species reactivity: guinea pig
611.00€ *
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Anti-IgG (H&L) X-adsorbed, Peroxidase conjugated
Anti-IgG (H&L) X-adsorbed, Peroxidase conjugated

Item number: I1903-06.1

USBiological
Applications: , Suitable for use in ELISA, Western Blot, Immunoperoxidase electron microscopy and Immunohistochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:25,000-1:125,000, Optimal dilutions to be determined by the researcher. Label: Horseradish Peroxidase (HRP), Storage and Stability:...
Application: ELISA, IHC, WB
Host: Goat
Species reactivity: hamster
858.00€ *
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Anti-IgG, Rhodamine conjugated
Anti-IgG, Rhodamine conjugated

Item number: I1903-08J.1

USBiological
Applications:, Suitable for use in ELISA and Immunofluorescence. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are...
Application: ELISA, IF, WB
Host: Rabbit
Species reactivity: mouse
755.00€ *
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Anti-IgG F(c) Peroxidase conjugated
Anti-IgG F(c) Peroxidase conjugated

Item number: I1904-25K.2

USBiological
Applications: , Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: ELISA: 1:6000-1:50,000. 1ug of canine IgG was used in a standard capture ELISA with ABTS (A0030) as a substrate for 30 minutes at RT., Western Blot: 1:100-1:10,000,...
Application: ELISA, IHC, WB
Host: Goat
Species reactivity: dog
675.00€ *
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Anti-IgM F(c) Biotin conjugated
Anti-IgM F(c) Biotin conjugated

Item number: I1905-01M.1500

USBiological
Applications:, Suitable for use in ELISA, Western Blot, Immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency. Other applications not tested. Recommended Dilution: ELISA: 1:40,000-1:190,000, using Peroxidase Conjugated...
Application: ELISA, IHC, WB
Host: Rabbit
Species reactivity: human
675.00€ *
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Anti-IgM, FITC conjugated
Anti-IgM, FITC conjugated

Item number: I1905-01Z.1500

USBiological
Applications:, Suitable for use in FLISA, Immunofluorescence and Fluorescent Western Blot.. Other applications have not been tested. Recommended Dilutions: FLISA: 1:10,000-1:50,000, Immunofluorescence: 1:1000-1:5000, Optimal dilutions to be determined by the researcher. Absorption Wavelength: 495nm, Emission...
Application: FLISA, IF, WB
Host: Rabbit
Species reactivity: mouse
596.00€ *
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Anti-Ig (human) Kappa Light Chain Purified, clone MEM-09 (Monoclonal) [Mouse]
Anti-Ig (human) Kappa Light Chain Purified, clone MEM-09...

Item number: MON-MON23249

MONOSAN
Immunoglobulin classes share the same basic four polypeptide chain structure of two heavy chains (five heavy chains types) and two light chains (kappa, lambda, both having a molecular weight of 22.5kDa). Kappa and lambda consist of a variable region and a constant region and can easily be differentiated by the...
Application: FC, IP, WB, IHC (paraffin), ICC, ELISA
Host: Mouse
Species reactivity: human
284.00€ *
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Anti-IgD (human) Purified, clone IA6-2 (Monoclonal) [Mouse]
Anti-IgD (human) Purified, clone IA6-2 (Monoclonal) [Mouse]

Item number: MON-MON23251

MONOSAN
Immunoglobulin D (IgD) is expressed on the surface of naive mature B cells, thus later than IgM, and is coexpressed with it then. Triggered by antigen binding, it signals through the CD79 complex to activate the B cells. Expression of IgD is lost after the isotype switch. Soluble IgD is present in very small amounts...
Application: FC
Host: Mouse
Species reactivity: human
218.00€ *
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650 from 659 pages
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